ArticleDevelopment (Cambridge, England)2014
PI(4,5)P2 regulates myoblast fusion through Arp2/3 regulator localization at the fusion site.
Article in Development (Cambridge, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- ARP2/3 protein complex regulates myoblast proliferation, morphology, migration, and fusion during embryonic chick skeletal myogenesis.In vitro cellular & developmental biology. Animal · 2026Article
- Membrane Ruffles: Composition, Function, Formation and Visualization.International journal of molecular sciences · 2024Review
- MTM1-mediated production of phosphatidylinositol 5-phosphate fuels the formation of podosome-like protrusions regulating myoblast fusion.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- BIN1 regulates actin-membrane interactions during IRSp53-dependent filopodia formation.Communications biology · 2024Article
- Transient inhibition of lysosomal functions potentiates nucleic acid vaccines.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Regulation of the myoblast fusion reaction for muscle development, regeneration, and adaptations.Experimental cell research · 2022Article
- Review
- An insight on Drosophila myogenesis and its assessment techniques.Molecular biology reports · 2020Review
- A SAC Phosphoinositide Phosphatase Controls Rice Development via Hydrolyzing PI4P and PI(4,5)PPlant physiology · 2020Article
- Review
- The fusogenic synapse at a glance.Journal of cell science · 2019Review
- How cells fuse.The Journal of cell biology · 2019Review
- Entosis Controls a Developmental Cell Clearance in C. elegans.Cell reports · 2019Article
- An RNAi based screen in Drosophila larvae identifies fascin as a regulator of myoblast fusion and myotendinous junction structure.Skeletal muscle · 2018Article
- Acting on identity: Myoblast fusion and the formation of the syncytial muscle fiber.Seminars in cell & developmental biology · 2017Review
- Real-time insights into regulated exocytosis.Journal of cell science · 2017Review
- Finding your inner modeler: An NSF-sponsored workshop to introduce cell biologists to modeling/computational approaches.Cellular logistics · 2017Article
- Article
- Arp2/3-mediated F-actin formation controls regulated exocytosis in vivo.Nature communications · 2015Article
- Membrane fusion in muscle development and repair.Seminars in cell & developmental biology · 2015Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Cell-cell fusion is a regulated process that requires merging of the opposing membranes and underlying cytoskeletons. However, the integration between membrane and cytoskeleton signaling during fusion is not known. Using Drosophila, we demonstrate that the membrane phosphoinositide PI(4,5)P2 is a crucial regulator of F-actin dynamics during myoblast fusion. PI(4,5)P2 is locally enriched and colocalizes spatially and temporally with the F-actin focus that defines the fusion site. PI(4,5)P2 enrichment depends on receptor engagement but is upstream or parallel to actin remodeling. Regulators of actin branching via Arp2/3 colocalize with PI(4,5)P2 in vivo and bind PI(4,5)P2 in vitro. Manipulation of PI(4,5)P2 availability leads to impaired fusion, with a reduction in the F-actin focus size and altered focus morphology. Mechanistically, the changes in the actin focus are due to a failure in the enrichment of actin regulators at the fusion site. Moreover, improper localization of these regulators hinders expansion of the fusion interface. Thus, PI(4,5)P2 enrichment at the fusion site encodes spatial and temporal information that regulates fusion progression through the localization of activators of actin polymerization.
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Registered trials
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