ArticleJournal of experimental botany2014
Arabidopsis thaliana phytochelatin synthase 2 is constitutively active in vivo and can rescue the growth defect of the PCS1-deficient cad1-3 mutant on Cd-contaminated soil.
Article in Journal of experimental botany, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 86 citations in OpenAlex.
- An Aqueous Extract ofInternational journal of molecular sciences · 2026Article
- Adaptive Plasticity of Phytochelatin Synthase Under Chromium Stress and Sulfur Availability inPlants (Basel, Switzerland) · 2026Article
- Phytoremediation capacity of tomato against Sb (III) focusing on the AsA/GSH cycle.Frontiers in plant science · 2026Article
- Overexpression of NTPCS1 Enhances Zn Tolerance in Tobacco.Plants (Basel, Switzerland) · 2025Article
- Phytochelatins and Cadmium Mitigation: Harnessing Genetic Avenues for Plant Functional Manipulation.International journal of molecular sciences · 2025Review
- Ancient duplication and functional differentiation of phytochelatin synthases is conserved in plant genomes.Horticulture research · 2025Article
- Phenylmercury stress induces root tip swelling through auxin homeostasis disruption.Plant molecular biology · 2024Article
- Phytochelatins Bind Zn(II) with Micro- to Picomolar Affinities without the Formation of Binuclear Complexes, Exhibiting Zinc Buffering and Muffling Rather than Storing Functions.Inorganic chemistry · 2024Article
- Characterization of aFrontiers in plant science · 2024Article
- Effect of phosphate on arsenic species uptake in plants under hydroponic conditions.Journal of plant research · 2023Article
- The Effect of Cadmium on Plants in Terms of the Response of Gene Expression Level and Activity.Plants (Basel, Switzerland) · 2023Review
- The Effect of Cadmium Tolerant Plant Growth Promoting Rhizobacteria on Plant Growth Promotion and Phytoremediation: A Review.Current microbiology · 2023Review
- Phytochelatins: Sulfur-Containing Metal(loid)-Chelating Ligands in Plants.International journal of molecular sciences · 2023Review
- Exogenously Applied Sodium Nitroprusside Mitigates Lead Toxicity in Rice by Regulating Antioxidants and Metal Stress-Related Transcripts.International journal of molecular sciences · 2022Article
- Phytochelatin-mediated metal detoxification pathway is crucial for an organomercurial phenylmercury tolerance in Arabidopsis.Plant molecular biology · 2022Article
- Heterologous Expression of the Phytochelatin SynthaseInternational journal of environmental research and public health · 2022Article
- Cytosolic phosphofructokinases are important for sugar homeostasis in leaves of Arabidopsis thaliana.Annals of botany · 2022Article
- Mesophyll specific expression of a bacterial mercury transporter-based vacuolar sequestration machinery sufficiently enhances mercury tolerance of Arabidopsis.Frontiers in plant science · 2022Article
- Molecular mechanism of Cu metal and drought stress resistance triggered byFrontiers in plant science · 2022Article
- The Phosphofructokinase Isoform AtPFK5 Is a Novel Target of Plastidic Thioredoxin-f-Dependent Redox Regulation.Antioxidants (Basel, Switzerland) · 2021Article
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4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phytochelatins play a key role in the detoxification of metals in plants and many other eukaryotes. Their formation is catalysed by phytochelatin synthases (PCS) in the presence of metal excess. It appears to be common among higher plants to possess two PCS genes, even though in Arabidopsis thaliana only AtPCS1 has been demonstrated to confer metal tolerance. Employing a highly sensitive quantification method based on ultraperformance electrospray ionization quadrupole time-of-flight mass spectrometry, we detected AtPCS2-dependent phytochelatin formation. Overexpression of AtPCS2 resulted in constitutive phytochelatin accumulation, i.e. in the absence of metal excess, both in planta and in a heterologous system. This indicates distinct enzymatic differences between AtPCS1 and AtPCS2. Furthermore, AtPCS2 was able to partially rescue the Cd hypersensitivity of the AtPCS1-deficient cad1-3 mutant in a liquid seedling assay, and, more importantly, when plants were grown on soil spiked with Cd to a level that is close to what can be found in agricultural soils. No rescue was found in vertical-plate assays, the most commonly used method to assess metal tolerance. Constitutive AtPCS2-dependent phytochelatin synthesis suggests a physiological role of AtPCS2 other than metal detoxification. The differences observed between wild-type plants and cad1-3 on Cd soil demonstrated: (i) the essentiality of phytochelatin synthesis for tolerating levels of Cd contamination that can naturally be encountered by plants outside of metal-rich habitats, and (ii) a contribution to Cd accumulation under these conditions.
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