ArticleJournal of experimental botany2014
Scopoletin is a phytoalexin against Alternaria alternata in wild tobacco dependent on jasmonate signalling.
Article in Journal of experimental botany, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 141 citations in OpenAlex.
- Simultaneous Overexpression of FERULOYL-CoA 6'-HYDROXYLASE 1 and COUMARIN SYNTHASE Leads to Coumarin-Enriched Lignin and Improved Saccharification in Greenhouse- and Field-Grown Poplar.Plant biotechnology journal · 2026Article
- Metabolomic and Transcriptomic Responses ofPlants (Basel, Switzerland) · 2026Article
- Metabolomics reveals McF6'H1-mediated regulation of phenylpropanoid/flavonoid metabolism and enhanced antifungal activity in transgenic Morinda citrifolia.BMC plant biology · 2026Article
- An Overview of theJournal of fungi (Basel, Switzerland) · 2026Review
- Metabolomic Insights into MYMV Resistance: Biochemical Complexity in Mung Bean Cultivars.Pathogens (Basel, Switzerland) · 2025Article
- The defensin gene NaDLP1, which confers resistance against Alternaria alternata, is transcriptionally regulated by NaWRKY3/6 in Nicotiana attenuata.BMC plant biology · 2025Article
- Microminutinin, a Fused Bis-Furan Coumarin fromPlants (Basel, Switzerland) · 2025Article
- Phenolic Compounds Accumulation and Cell Death Degree Induced by Fusaric Acid in Agroforestry Hosts Plants ofJournal of fungi (Basel, Switzerland) · 2025Article
- Cooperation between a root fungal endophyte and host-derived coumarin scopoletin mediates Arabidopsis iron nutrition.The New phytologist · 2025Article
- Decursin, Identified via High-Throughput Chemical Screening, Enhances Plant Disease Resistance via Two Independent Mechanisms.Molecular plant pathology · 2025Article
- β-Carotene alleviates substrate inhibition caused by asymmetric cooperativity.Nature communications · 2025Article
- Novel Regulators and Their Epistatic Networks in Arabidopsis' Defence Responses to Alternaria alternata Infection.Molecular plant pathology · 2025Article
- Coumarin Promotes Hypocotyl Elongation by Increasing the Synthesis of Brassinosteroids in Plants.International journal of molecular sciences · 2025Article
- Advances in WRKY regulation of immune responses in medicinal plants.Frontiers in plant science · 2025Review
- The biosynthetic pathway of coumarin and its genetic regulation in response to biotic and abiotic stresses.Frontiers in plant science · 2025Review
- What are the 100 most cited fungal genera?Studies in mycology · 2024Article
- Transcriptome responses of Arabidopsis to necrotrophic fungus Alternaria brassicae reveal pathways and candidate genes associated with resistance.Plant molecular biology · 2024Article
- Combined metabolome and transcriptome reveal HmF6'H1 regulating simple coumarin accumulation against powdery mildew infection in Heracleum moellendorffii Hance.BMC plant biology · 2024Article
- Resistance toPlants (Basel, Switzerland) · 2024Article
- Synergistic induction of phytoalexins in Nicotiana attenuata by jasmonate and ethylene signaling mediated by NaWRKY70.Journal of experimental botany · 2024Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alternaria alternata (tobacco pathotype) is a necrotrophic fungus causing severe losses in Nicotiana species by infection of mature leaves. Similar to what has been observed in cultivated tobacco, N. tabacum, young leaves of wild tobacco, N. attenuata, were more resistant to A. alternata than mature leaves, and this was correlated with stronger blue fluorescence induced after infection. However, the nature of the fluorescence-emitting compound, its role in defence, and its regulation were not clear. Silencing feruloyl-CoA 6'-hydroxylase 1 (F6'H1), the gene encoding the key enzyme for scopoletin biosynthesis, by virus-induced gene silencing (VIGS) revealed that the blue fluorescence was mainly emitted by scopoletin and its β-glycoside form, scopolin. Further analysis showed that scopoletin exhibited strong antifungal activity against A. alternata in vitro and in vivo. Importantly, jasmonic acid (JA) levels were highly elicited in young leaves but much less in mature leaves after infection; and fungus-elicited scopoletin was absent in JA-deficient plants, but was largely restored with methyl jasmonate treatments. Consistent with this, plants strongly impaired in JA biosynthesis and perception were highly susceptible to A. alternata in the same way scopoletin/scopolin-depleted VIGS F6'H1 plants. Furthermore, silencing MYC2, a master regulator of most JA responses, reduced A. alternata-induced NaF6'H1 transcripts and scopoletin. Thus, it is concluded that JA signalling is activated in N. attenuata leaves after infection, which subsequently regulates scopoletin biosynthesis for the defence against A. alternata partly through MYC2, and higher levels of scopoletin accumulated in young leaves account for their strong resistance.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.