ArticleProceedings of the National Academy of Sciences of the United States of America2014
Highly efficient integration and expression of piggyBac-derived cassettes in the honeybee (Apis mellifera).
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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62 citing papers in PubMed, 128 citations in OpenAlex.
- Disruption of yellow-e3 impairs both adult molting and cuticular melanization in the honeybee (Apis mellifera).Frontiers in zoology · 2026Article
- Emerging tools to advance neuroethology in butterflies and moths.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2026Review
- The fru gene specifies male cooperative behaviors in honeybee colonies.Nature communications · 2025Article
- Long-term fluorescence live imaging of honeybee embryos using light sheet fluorescence microscopy and halocarbon-based liquids.Biology open · 2025Article
- Manipulation of a New Non-model Insect Genome Using Targeted CRISPR-Era Approaches.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Dedicated developmental programing for group-supporting behaviors in eusocial honeybees.Science advances · 2024Article
- Knockout of a single Pax6 gene (toy but not ey) leads to compound eye deficiency and small head in honeybees.Communications biology · 2024Article
- Transcriptomic Analysis of Genes Associated with Stinger Development at Different Life Stages ofInternational journal of molecular sciences · 2024Article
- A perspective on neuroethology: what the past teaches us about the future of neuroethology.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2024Review
- Recognition of polymorphic Csd proteins determines sex in the honeybee.Science advances · 2023Article
- Honey bee functional genomics using symbiont-mediated RNAi.Nature protocols · 2023Review
- The function and evolution of a genetic switch controlling sexually dimorphic eye differentiation in honeybees.Nature communications · 2023Article
- Multisite imaging of neural activity using a genetically encoded calcium sensor in the honey bee.PLoS biology · 2023Article
- Highly efficient site-specific integration of DNA fragments into the honeybee genome using CRISPR/Cas9.G3 (Bethesda, Md.) · 2022Article
- In-vivo egfp expression in the honeybee Apis mellifera induced by electroporation and viral expression vector.PloS one · 2022Article
- Genetic and viral approaches to record or manipulate neurons in insects.Current opinion in insect science · 2021Review
- Article
- A molecular toolkit for superorganisms.Trends in genetics : TIG · 2021Review
- Societies to genes: can we get there from here?Genetics · 2021Article
- Immune Response of Eastern Honeybee Worker toInsects · 2021Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Honeybees (Apis mellifera), which are important pollinators of plants, display remarkable individual behaviors that collectively contribute to the organization of a complex society. Advances in dissecting the complex processes of honeybee behavior have been limited in the recent past due to a lack of genetic manipulation tools. These tools are difficult to apply in honeybees because the unit of reproduction is the colony, and many interesting phenotypes are developmentally specified at later stages. Here, we report highly efficient integration and expression of piggyBac-derived cassettes in the honeybee. We demonstrate that 27 and 20% of queens stably transmitted two different expression cassettes to their offspring, which is a 6- to 30-fold increase in efficiency compared with those generally reported in other insect species. This high efficiency implies that an average beekeeping facility with a limited number of colonies can apply this tool. We demonstrated that the cassette stably and efficiently expressed marker genes in progeny under either an artificial or an endogenous promoter. This evidence of efficient expression encourages the use of this system to inhibit gene functions through RNAi in specific tissues and developmental stages by using various promoters. We also showed that the transgenic marker could be used to select transgenic offspring to be employed to facilitate the building of transgenic colonies via the haploid males. We present here the first to our knowledge genetic engineering tool that will efficiently allow for the systematic detection and better understanding of processes underlying the biology of honeybees.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.