ArticleProceedings of the National Academy of Sciences of the United States of America2014
Site-specific genetic engineering of the Anopheles gambiae Y chromosome.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed.
- Sex distorter male drive for resistance-resilient population control of the human malaria vector Anopheles gambiae.Nature communications · 2026Article
- Functional validation of a white pupae minimal gene construct in Ceratitis capitata (Diptera: Tephritidae).Insect science · 2026Article
- Genetic engineering for SIT application: a fruit fly-focused review.Insect science · 2026Review
- A male-drive female-sterile system for the self-limited control of the malaria mosquito Anopheles gambiae.Nature communications · 2025Article
- CRISPR technologies for the control and study of malaria-transmitting anopheline mosquitoes.Parasites & vectors · 2025Review
- Engineering drive-selection balance for localized population suppression with neutral dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- A Y chromosome-linked genome editor for efficient population suppression in the malaria vector Anopheles gambiae.Nature communications · 2025Article
- Establishing a dominant early larval sex-selection strain in the Asian malaria vector Anopheles stephensi.Infectious diseases of poverty · 2024Article
- Eliminating malaria vectors with precision-guided sterile males.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Targeting mosquito X-chromosomes reveals complex transmission dynamics of sex ratio distorting gene drives.Nature communications · 2024Article
- Y chromosome shredding in Anopheles gambiae: Insight into the cellular dynamics of a novel synthetic sex ratio distorter.PLoS genetics · 2024Article
- Article
- Efficient sex separation by exploiting differential alternative splicing of a dominant marker in Aedes aegypti.PLoS genetics · 2023Article
- Article
- Eliminating Malaria Vectors with Precision Guided Sterile Males.bioRxiv : the preprint server for biology · 2023Article
- Article
- A confinable female-lethal population suppression system in the malaria vector,Science advances · 2023Article
- Combining two genetic sexing strains allows sorting of non-transgenic males for Aedes genetic control.Communications biology · 2023Article
- The salivary protein Saglin facilitates efficient midgut colonization of Anopheles mosquitoes by malaria parasites.PLoS pathogens · 2023Article
- Gene drive designs for efficient and localisable population suppression using Y-linked editors.PLoS genetics · 2022Article
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Authors and funding
8 authors.
Funding
Abstract
Despite its function in sex determination and its role in driving genome evolution, the Y chromosome remains poorly understood in most species. Y chromosomes are gene-poor, repeat-rich and largely heterochromatic and therefore represent a difficult target for genetic engineering. The Y chromosome of the human malaria vector Anopheles gambiae appears to be involved in sex determination although very little is known about both its structure and function. Here, we characterize a transgenic strain of this mosquito species, obtained by transposon-mediated integration of a transgene construct onto the Y chromosome. Using meganuclease-induced homologous repair we introduce a site-specific recombination signal onto the Y chromosome and show that the resulting docking line can be used for secondary integration. To demonstrate its utility, we study the activity of a germ-line-specific promoter when located on the Y chromosome. We also show that Y-linked fluorescent transgenes allow automated sex separation of this important vector species, providing the means to generate large single-sex populations. Our findings will aid studies of sex chromosome function and enable the development of male-exclusive genetic traits for vector control.
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