ArticleBiochimica et biophysica acta2014
Glucose starvation-induced turnover of the yeast glucose transporter Hxt1.
Article in Biochimica et biophysica acta, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Do seconds make a difference? Investigating strain-specific behavior of yeast in dynamic glucose environments.Microbial cell factories · 2026Article
- Dynamic patterns of gene expression match extracellular signals through push-pull regulation.PLoS genetics · 2025Article
- Integrated transcriptomics and metabolomics analysis reveal the regulatory mechanisms underlying the combined effects of heat and glucose starvation on carotenoid biosynthesis in Rhodotorula glutinis YM25079.Biotechnology for biofuels and bioproducts · 2025Article
- Expression of a periplasmic β-glucosidase from Yarrowia lipolytica allows efficient cellobiose-xylose co-fermentation by industrial xylose-fermenting Saccharomyces cerevisiae strains.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Molecular Modification Enhances Xylose Uptake by the Sugar Transporter KM_SUT5 ofInternational journal of molecular sciences · 2024Article
- Multiple roles for the cytoplasmic C-terminal domains of the yeast cell surface receptors Rgt2 and Snf3 in glucose sensing and signaling.Scientific reports · 2024Article
- The role of salt bridge networks in the stability of the yeast hexose transporter 1.Biochimica et biophysica acta. General subjects · 2023Article
- Casein kinases are required for the stability of the glucose-sensing receptor Rgt2 in yeast.Scientific reports · 2022Article
- Strategies for Efficient Expression of Heterologous Monosaccharide Transporters inJournal of fungi (Basel, Switzerland) · 2022Article
- Fission Yeast TORC2 Signaling Pathway Ensures Cell Proliferation under Glucose-Limited, Nitrogen-Replete Conditions.Biomolecules · 2021Review
- Identification of key residues for efficient glucose transport by the hexose transporter CgHxt4 in high sugar fermentation yeast Candida glycerinogenes.Applied microbiology and biotechnology · 2021Article
- Role of Calcium/Calcineurin Signalling in Regulating Intracellular Reactive Oxygen Species Homeostasis inGenes · 2021Article
- Effects ofMolecular medicine reports · 2021Article
- 'Sugarcoating' 2-deoxyglucose: mechanisms that suppress its toxic effects.Current genetics · 2021Review
- Endocytosis of nutrient transporters in fungi: The ART of connecting signaling and trafficking.Computational and structural biotechnology journal · 2021Review
- Novel xylose transporter Cs4130 expands the sugar uptake repertoire in recombinantBiotechnology for biofuels · 2020Article
- Asymmetric distribution of glucose transporter mRNA provides a growth advantage in yeast.The EMBO journal · 2019Article
- Engineering of Pentose Transport inFrontiers in bioengineering and biotechnology · 2019Review
- Glucose repression can be alleviated by reducing glucose phosphorylation rate in Saccharomyces cerevisiae.Scientific reports · 2018Article
- Role for ribosome-associated complex and stress-seventy subfamily B (RAC-Ssb) in integral membrane protein translation.The Journal of biological chemistry · 2017Article
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4 authors.
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Abstract
backgroundThe budding yeast Saccharomyces cerevisiae possesses multiple glucose transporters with different affinities for glucose that enable it to respond to a wide range of glucose concentrations. The steady-state levels of glucose transporters are regulated in response to changes in the availability of glucose. This study investigates the glucose regulation of the low affinity, high capacity glucose transporter Hxt1. METHODS AND
resultsWestern blotting and confocal microscopy were performed to evaluate glucose regulation of the stability of Hxt1. Our results show that glucose starvation induces endocytosis and degradation of Hxt1 and that this event requires End3, a protein required for endocytosis, and the Doa4 deubiquitination enzyme. Mutational analysis of the lysine residues in the Hxt1 N-terminal domain demonstrates that the two lysine residues, K12 and K39, serve as the putative ubiquitin-acceptor sites by the Rsp5 ubiquitin ligase. We also demonstrate that inactivation of PKA (cAMP-dependent protein kinase A) is needed for Hxt1 turnover, implicating the role of the Ras/cAMP-PKA glucose signaling pathway in the stability of Hxt1. CONCLUSION AND GENERAL SIGNIFICANCE: Hxt1, most useful when glucose is abundant, is internalized and degraded when glucose becomes depleted. Of note, the stability of Hxt1 is regulated by PKA, known as a positive regulator for glucose induction of HXT1 gene expression, demonstrating a dual role of PKA in regulation of Hxt1.
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