ArticleThe Indian journal of medical research2014
A cfr-positive clinical staphylococcal isolate from India with multiple mechanisms of linezolid-resistance.
Article in The Indian journal of medical research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- Linezolid MIC creep among clinically significantFrontiers in antibiotics · 2026Article
- Diversity in Adaptive Evolution of Methicillin-ResistantInfection and drug resistance · 2025Article
- Recent development and fighting strategies for lincosamide antibiotic resistance.Clinical microbiology reviews · 2024Review
- Detection of a Novel G2603T Mutation in cfr Harboring Linezolid-ResistantJournal of laboratory physicians · 2023Article
- Mucoid Staphylococcus haemolyticus: an unheeded multidrug-resistant pathogen.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Clinical Infections, Antibiotic Resistance, and Pathogenesis ofMicroorganisms · 2022Review
- Mobile Oxazolidinone Resistance Genes in Gram-Positive and Gram-Negative Bacteria.Clinical microbiology reviews · 2021Article
- First Case of Staphylococci Carrying Linezolid Resistance Genes from Laryngological Infections in Poland.Pathogens (Basel, Switzerland) · 2021Article
- Linezolid resistant coagulase negative staphylococci (LRCoNS) with novel mutations causing blood stream infections (BSI) in India.BMC infectious diseases · 2019Article
- Characterization of Vancomycin Resistant Enterococci in Hospitalized Patients and Role of Gut Colonization.Journal of clinical and diagnostic research : JCDR · 2017Article
- Linezolid-resistant mucoid Staphylococcus haemolyticus from a tertiary-care centre in Delhi.New microbes and new infections · 2016Article
- Emergence of cfr-Mediated Linezolid Resistance in a Methicillin-Resistant Staphylococcus aureus Epidemic Clone Isolated from Patients with Cystic Fibrosis.Antimicrobial agents and chemotherapy · 2015Article
- Detection of a New cfr-Like Gene, cfr(B), in Enterococcus faecium Isolates Recovered from Human Specimens in the United States as Part of the SENTRY Antimicrobial Surveillance Program.Antimicrobial agents and chemotherapy · 2015Article
- Mutations in the bacterial ribosomal protein l3 and their association with antibiotic resistance.Antimicrobial agents and chemotherapy · 2015Article
- Linezolid-resistant Staphylococcus aureus strain 1128105, the first known clinical isolate possessing the cfr multidrug resistance gene.Antimicrobial agents and chemotherapy · 2014Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND &
objectivesLinezolid, a member of the oxazolidinone class of antibiotics, has been an effective therapeutic option to treat severe infections caused by multidrug resistant Gram positive bacteria. Emergence of linezolid resistant clinical strains is a serious issue in the healthcare settings worldwide. We report here the molecular characterization of a linezolid resistant clinical isolate of Staphylococcus haemolyticus from India.
methodsThe species of the clinical isolate was identified by 16S rRNA gene sequencing. The minimum inhibitory concentrations (MICs) of linezolid, clindamycin, chloramphenicol and oxacillin were determined by E-test method. To elucidate the mechanism of linezolid-resistance, presence of cfr gene (chloramphenicol florfenicol resistance) and mutations in 23S rRNA and ribosomal proteins (L3, L4 and L22) were investigated. Staphylococcal Cassette Chromosome mec (SCCmec) typing was performed by multiplex PCR.
resultsThe study documented a rare clinical S. haemolyticus strain with three independent mechanisms of linezolid-resistance. The strain carried cfr gene, the only known transmissible mechanism of linezolid-resistance. The strain also possessed resistance-conferring mutations such as G 2576 T in domain V of 23S rRNA gene and Met 156 Thr in L3 ribosomal protein. The other ribosomal proteins (L4 and L22) did not exhibit mutations accountable for linezolid-resistance. Restriction digestion by NheI revealed that all the alleles of 23S rRNA gene were mutated. The isolate showed elevated MIC values (>256 μg ml -[1] of linezolid, clindamycin, chloramphenicol and oxacillin. Methicillin resistance was conferred by type I SCCmec element. The strain also harboured lsa(B) gene which encodes an ABC transporter that can efflux clindamycin. INTERPRETATION &
conclusionsThe present study reports the first clinical strain from India with transmissible and multiple mechanisms of linezolid-resistance. Judicious use of linezolid in clinical practice and proper surveillance of cfr-positive strains are of utmost importance to safeguard the efficacy of linezolid.
Indexed as
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24820843PMC4069743W1700561774What OpenQuestion holds
Registered trials
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