ArticleMolecular and cellular biology2014
The metazoan-specific mediator subunit 26 (Med26) is essential for viability and is found at both active genes and pericentric heterochromatin in Drosophila melanogaster.
Article in Molecular and cellular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- Expanding the HP1a-binding consensus and molecular grammar for heterochromatin assembly.Nucleic acids research · 2025Article
- MLE/DHX9 Helicase Activity Is Required to Regulate the Expression of a Number of Tissue-Specific Genes on Chromosome 4 in Drosophila melanogaster.Doklady. Biochemistry and biophysics · 2025Article
- Article
- NetREX-CF integrates incomplete transcription factor data with gene expression to reconstruct gene regulatory networks.Communications biology · 2022Article
- Poly(ADP-ribose) polymerase 1 in genome-wide expression control in Drosophila.Scientific reports · 2020Article
- Insights into HP1a-Chromatin Interactions.Cells · 2020Review
- Controlling the Master: Chromatin Dynamics at the MYC Promoter Integrate Developmental Signaling.Genes · 2017Review
- Cbx3 maintains lineage specificity during neural differentiation.Genes & development · 2017Article
- Defining the essential function of FBP/KSRP proteins: Drosophila Psi interacts with the mediator complex to modulate MYC transcription and tissue growth.Nucleic acids research · 2016Article
- Evolution of disorder in Mediator complex and its functional relevance.Nucleic acids research · 2016Article
- The Mediator complex: a central integrator of transcription.Nature reviews. Molecular cell biology · 2015Review
- MED26 regulates the transcription of snRNA genes through the recruitment of little elongation complex.Nature communications · 2015Article
- Reconstitution of active human core Mediator complex reveals a critical role of the MED14 subunit.Nature structural & molecular biology · 2014Article
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4 authors at 3 institutions in 1 country.
Funding
Abstract
Human MED26 was originally purified in the cofactor required for the Sp1 activation complex (CRSP) as a 70-kDa component named CRSP70. This polypeptide was specific to metazoans and the “small” form of the Mediator complex. We report here that a Drosophila melanogaster homologue of MED26 similarly interacts with other components of the core Drosophila Mediator complex but not with the kinase module and is recruited to genes upon activation. Using a null allele of Med26, we show that Med26 is required for organismal viability but not for cell proliferation or survival. Clones lacking Med26 in the wing disc lead to loss of the adult wing margin and reduced expression of genes involved in wing margin formation. Surprisingly, when polytene chromosomes from the salivary gland were examined using antibodies to Med26, it was apparent that a fraction of the protein was associated with the chromocenter, which contains pericentric heterochromatin. This staining colocalizes with heterochromatin protein 1 (HP1). Immunoprecipitation experiments show that Med26 interacts with HP1. The interaction is mediated through the chromoshadow domain of HP1 and through the conserved motif in the carboxy terminus of the Med26 protein. This work is the first characterization of the metazoan-specific Mediator subunit in an animal model.
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