ArticlePlant cell reports2014
Selection of suitable soybean EF1α genes as internal controls for real-time PCR analyses of tissues during plant development and under stress conditions.
Article in Plant cell reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed.
- Maintaining sulfur supply to the symbiosome delays nodule senescence in soybean.Nature communications · 2025Article
- Characterizing reference genes for high-fidelity gene expression analysis under different abiotic stresses and elicitor treatments in fenugreek leaves.Plant methods · 2024Article
- Selection and Validation of Reference Genes for Reverse-Transcription Quantitative PCR Analysis inInternational journal of molecular sciences · 2023Article
- Comprehensive and evolutionary analysis ofFrontiers in plant science · 2023Article
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- Reference gene selection for quantitative RT-PCR in Miscanthus sacchariflorus under abiotic stress conditions.Molecular biology reports · 2022Article
- Using the Knowledge of Post-transcriptional Regulations to Guide Gene Selections for Molecular Breeding in Soybean.Frontiers in plant science · 2022Review
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- Selection and Validation of Reference Genes for Quantitative Real-Time PCR in White Clover (Plants (Basel, Switzerland) · 2020Article
- Gene expression during development and overexpression after Cercospora kikuchii and salicylic acid challenging indicate defensive roles of the soybean toxin.Plant cell reports · 2020Article
- Identification and evaluation of reference genes for reliable normalization of real-time quantitative PCR data in acerola fruit, leaf, and flower.Molecular biology reports · 2020Article
- Selection and Validation of Appropriate Reference Genes for Quantitative RT-PCR Analysis inBioMed research international · 2020Article
- Differential expression of recently duplicated PTOX genes in Glycine max during plant development and stress conditions.Journal of bioenergetics and biomembranes · 2019Article
- Identification and functional characterization of the chloride channel gene, GsCLC-c2 from wild soybean.BMC plant biology · 2019Article
- Validation of Internal Control Genes for Quantitative Real-Time PCR Gene Expression Analysis inMolecules (Basel, Switzerland) · 2018Article
- Selection and Validation of Reference Genes for mRNA Expression by Quantitative Real-Time PCR Analysis in Neolamarckia cadamba.Scientific reports · 2018Article
- Reference gene selection for molecular studies of dormancy in wild oat (Avena fatua L.) caryopses by RT-qPCR method.PloS one · 2018Article
- Identification and Validation of Reference Genes for Seashore Paspalum Response to Abiotic Stresses.International journal of molecular sciences · 2017Article
- Stability evaluation of reference genes for gene expression analysis by RT-qPCR in soybean under different conditions.PloS one · 2017Article
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5 authors.
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Abstract
key messageThe EF1α genes were stable in the large majority of soybean tissues during development and in specific tissues/conditions under stress. Quantitative real-time PCR (qPCR) analysis strongly depends on transcript normalization using stable reference genes. Reference genes are generally encoded by multigene families and are used in qPCR normalization; however, little effort has been made to verify the stability of different gene members within a family. Here, the expression stability of members of the soybean EF1α gene family (named EF1α 1a1, 1a2, 1b, 2a, 2b and 3) was evaluated in different tissues during plant development and stress exposure (SA and PEG). Four genes (UKN1, SKIP 16, EF1β and MTP) already established as stably expressed were also used in the comparative analysis. GeNorm analyses revealed different combinations of reference genes as stable in soybean tissues during development. The EF1α genes were the most stable in cotyledons (EF1α 3 and EF1α 1b), epicotyls (EF1α 1a2, EF1α 2b and EF1α 1a1), hypocotyls (EF1α 1a1 and EF1β), pods (EF1α 2a and EF1α 2b) and roots (EF1α 2a and UKN1) and less stable in tissues such as trifoliate and unifoliate leaves and germinating seeds. Under stress conditions, no suitable combination including only EF1α genes was found; however, some genes were relatively stable in leaves (EF1α 1a2) and roots (EF1α 1a1) treated with SA as well as in roots treated with PEG (EF1α 2b). EF1α 2a was the most stably expressed EF1α gene in all soybean tissues under stress. Taken together, our data provide guidelines for the selection of EF1α genes for use as reference genes in qPCR expression analyses during plant development and under stress conditions.
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