ArticlePloS one2014
Effect of nicotine and porphyromonas gingivalis lipopolysaccharide on endothelial cells in vitro.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 2 registered trials, which are not on this map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Snuff Use, Smoking, Periodontal Health and Premature Death: 30-year Study
Snuff Use, Smoking, Periodontitis and Mortality: 30-year Cohort Study From Sweden
Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- Periodontal diseases among pregnant adolescents and young women in Nigeria: a scoping review.BMC oral health · 2025Article
- Vascularized liver-on-a-chip model to investigate nicotine-induced dysfunction.Biomicrofluidics · 2023Article
- Effects of nicotine and lipopolysaccharide stimulation on adhesion molecules in human gingival endothelial cells.Odontology · 2023Article
- The impact of periodontitis on vascular endothelial dysfunction.Frontiers in cellular and infection microbiology · 2022Review
- Effects of Electronic Nicotine Delivery Systems and Cigarettes on Systemic Circulation and Blood-Brain Barrier: Implications for Cognitive Decline.The American journal of pathology · 2021Review
- Potential Suppressive Effect of Nicotine on the Inflammatory Response in Oral Epithelial Cells: An In Vitro Study.International journal of environmental research and public health · 2021Article
- Tobacco Products, Periodontal Health and Education Level: Cohort Study from Sweden.Dentistry journal · 2020Article
- Electronic cigarette exposure disrupts blood-brain barrier integrity and promotes neuroinflammation.Brain, behavior, and immunity · 2020Article
- Crosstalk between Akt and NF-κB pathway mediates inhibitory effect of gas6 on monocytes-endothelial cells interactions stimulated by P. gingivalis-LPS.Journal of cellular and molecular medicine · 2020Article
- The Impact of Smoking on Subgingival Microflora: From Periodontal Health to Disease.Frontiers in microbiology · 2020Review
- Bacterial lipopolysaccharide and antimicrobial LL-37 enhance ICAM-1 expression and NF-κB p65 phosphorylation in senescent endothelial cells.International journal of molecular medicine · 2019Article
- Effect of tobacco on periodontal disease and oral cancer.Tobacco induced diseases · 2019Review
- Tobacco-induced suppression of the vascular response to dental plaque.Molecular oral microbiology · 2018Review
- Effect of tyrosine-rich amelogenin peptide on behavior and differentiation of endothelial cells.Clinical oral investigations · 2016Article
- Endothelial disruptive proinflammatory effects of nicotine and e-cigarette vapor exposures.American journal of physiology. Lung cellular and molecular physiology · 2015Article
- Article
- Platelet-Derived Biomaterials Inhibit Nicotine-Induced Intervertebral Disc Degeneration Through Regulating IGF-1/AKT/IRS-1 Signaling Axis.Cell transplantationArticle
- Amelioration of Nicotine-Induced Osteoarthritis by Platelet-Derived Biomaterials Through Modulating IGF-1/AKT/IRS-1 Signaling Axis.Cell transplantationArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Smoking is considered a significant risk factor for both periodontal disease and cardiovascular disease (CVD). Endothelial cells play an important role in the progression of both diseases. In the present study, we investigated in vitro the impact of nicotine on functional properties of human umbilical vein endothelial cells (HUVECs) stimulated with lipopolysaccharide (LPS) of periodontal pathogen Porphyromonas gingivalis. HUVECs were stimulated with different concentrations of nicotine (10 µM-10 mM) and/or P. gingivalis LPS. Expression levels of intercellular adhesion molecule-1, vascular cell adhesion molecule-1, E-selectin, monocyte chemoattractant protein 1, and interleukin-8 were measured on both gene and protein levels. Cell proliferation/viability, apoptosis, and migration were also investigated. Nicotine at a concentration of 10 mM significantly decreased P. gingivalis LPS-induced expression of all investigated proteins after 4 h stimulation, while lower nicotine concentrations had no significant effect on protein expression with or without P. gingivalis LPS. Proliferation/viability of HUVECs was also significantly inhibited by 10-mM nicotine but not by lower concentrations. Migration of HUVECs was significantly decreased by nicotine at concentrations of 1-10 mM. Nicotine at a concentration similar to that observed in the serum of smokers had no significant effect on the functional properties of HUVECs. However, high concentrations of nicotine, similar to that observed in the oral cavity of smokers, inhibited the inflammatory response of HUVECs. This effect of nicotine might be associated with decreased gingival bleeding indices in smoking periodontitis patients.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.