ArticleScientific reports2014
Recapitulating physiological and pathological shear stress and oxygen to model vasculature in health and disease.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it, 72 citations in OpenAlex.
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- Microtubules and mechanosensing: key players in endothelial responses to mechanical stimuli.Cellular and molecular life sciences : CMLS · 2025Review
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- Tension at the gate: sensing mechanical forces at the blood-brain barrier in health and disease.Journal of neuroinflammation · 2024Review
- Highly scalable and standardized organ-on-chip platform with TEER for biological barrier modeling.Tissue barriers · 2024Article
- Controlling Pericellular Oxygen Tension in Cell Culture Reveals Distinct Breast Cancer Responses to Low Oxygen Tensions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Chronic Opioid Treatment Arrests Neurodevelopment and Alters Synaptic Activity in Human Midbrain Organoids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Mimicking blood and lymphatic vasculatures using microfluidic systems.Biomicrofluidics · 2024Review
- Vascular protection afforded by zinc supplementation in human coronary artery smooth muscle cells mediated by NRF2 signaling under hypoxia/reoxygenation.Redox biology · 2023Article
- Modeling angiogenesis in the human brain in a tissue-engineered post-capillary venule.Angiogenesis · 2023Article
- Microfluidic platform for the reproduction of hypoxic vascular microenvironments.Scientific reports · 2023Article
- Extracellular vesicles produced by human-induced pluripotent stem cell-derived endothelial cells can prevent arterial stenosis in mice via autophagy regulation.Frontiers in cardiovascular medicine · 2022Article
- Harnessing biomaterials for lymphatic system modulation.Acta biomaterialia · 2021Review
- Transforming a well into a chip: A modular 3D-printed microfluidic chip.APL bioengineering · 2021Article
- Bacteriophage uptake by mammalian cell layers represents a potential sink that may impact phage therapy.iScience · 2021Article
- In Vitro/Ex Vivo Models for the Study of Ischemia Reperfusion Injury during Kidney Perfusion.International journal of molecular sciences · 2020Review
- From arteries to capillaries: approaches to engineering human vasculature.Advanced functional materials · 2020Article
- Collagen fiber structure guides 3D motility of cytotoxic T lymphocytes.Matrix biology : journal of the International Society for Matrix Biology · 2020Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Studying human vascular disease in conventional cell cultures and in animal models does not effectively mimic the complex vascular microenvironment and may not accurately predict vascular responses in humans. We utilized a microfluidic device to recapitulate both shear stress and O2 levels in health and disease, establishing a microfluidic vascular model (μVM). Maintaining human endothelial cells (ECs) in healthy-mimicking conditions resulted in conversion to a physiological phenotype namely cell elongation, reduced proliferation, lowered angiogenic gene expression and formation of actin cortical rim and continuous barrier. We next examined the responses of the healthy μVM to a vasotoxic cancer drug, 5-Fluorouracil (5-FU), in comparison with an in vivo mouse model. We found that 5-FU does not induce apoptosis rather vascular hyperpermeability, which can be alleviated by Resveratrol treatment. This effect was confirmed by in vivo findings identifying a vasoprotecting strategy by the adjunct therapy of 5-FU with Resveratrol. The μVM of ischemic disease demonstrated the transition of ECs from a quiescent to an activated state, with higher proliferation rate, upregulation of angiogenic genes, and impaired barrier integrity. The μVM offers opportunities to study and predict human ECs with physiologically relevant phenotypes in healthy, pathological and drug-treated environments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.