Evidence map›Paper›PMID 24818132›Full record

ArticleBioMed research international2014

Extracts from glioma tissues following cryoablation have proapoptosis, antiproliferation, and anti-invasion effects on glioma cells.

Tianzhu Liu, Xin Wang, Zhilin Yin, Jun Pan, Hongbo Guo, Shizhong Zhang

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BioMed research international, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Tianzhu LiuDepartment of Neurosurgery, Neurosurgery Institute of Guangdong Province, Key Laboratory on Brain Function Rebuild and Rehabilitation of Guangdong, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Xin WangDepartment of Neurosurgery, Neurosurgery Institute of Guangdong Province, Key Laboratory on Brain Function Rebuild and Rehabilitation of Guangdong, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Zhilin YinDepartment of Neurosurgery, Neurosurgery Institute of Guangdong Province, Key Laboratory on Brain Function Rebuild and Rehabilitation of Guangdong, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Jun PanDepartment of Neurosurgery, Neurosurgery Institute of Guangdong Province, Key Laboratory on Brain Function Rebuild and Rehabilitation of Guangdong, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Hongbo GuoDepartment of Neurosurgery, Neurosurgery Institute of Guangdong Province, Key Laboratory on Brain Function Rebuild and Rehabilitation of Guangdong, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Shizhong ZhangDepartment of Neurosurgery, Neurosurgery Institute of Guangdong Province, Key Laboratory on Brain Function Rebuild and Rehabilitation of Guangdong, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Southern Medical University · CNZhujiang Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveThis study is to investigate the in vivo apoptotic processes in glioma tissues following cryoablation and the effects of glioma tissue extracts on GL261 glioma cells in vitro.

methodsTUNEL and flow cytometry analysis were performed to detect the apoptotic processes in the glioma tissues following cryoablation and in the GL261 cells treated with cryoablated tumor extracts. The scratch assay, the transwell assay, and Western blot analysis were carried out to evaluate the effects of cryoablated tumor extracts on the migration, invasion, and proliferation of tumor cells.

resultsOur in vivo results indicated that the rapid-onset apoptosis was induced via the intrinsic pathway and the delayed apoptosis was triggered through the extrinsic pathway. The in vitro results showed that extracts from glioma tissues following cryoablation induced apoptosis via extrinsic pathways in GL261 glioma cells. Furthermore, cryoablated tumor extracts significantly inhibited the migration and proliferation of these cells, which would be related to the inhibition of ERK1/2 pathway and the activation of P38 pathway.

conclusionGlioma cells surviving in cryoablation undergo intrinsic or extrinsic apoptosis. Augmenting the induction of apoptosis or enhancing the cryosensitization of tumor cells by coupling cryoablation with specific chemotherapy effectively increases the efficiency of this therapeutic treatment.

Indexed as

AnimalsApoptosisBrain NeoplasmsCell Line, TumorCell MovementCell ProliferationCryosurgeryExtracellular Signal-Regulated MAP KinasesFemaleGliomaHumansMaleMiceNeoplasm Invasivenessp38 Mitogen-Activated Protein KinasesSignal TransductionExtracellular Signal-Regulated MAP Kinasesp38 Mitogen-Activated Protein KinasesTissue Extracts

Identifiers

PMID24818132
PMCPMC4004080
OpenAlexW2007889786

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.