Evidence map›Paper›PMID 24817993›Full record

ArticleOxidative medicine and cellular longevity2014

Endogenous ceramide contributes to the transcytosis of oxLDL across endothelial cells and promotes its subendothelial retention in vascular wall.

Wenjing Li, Xiaoyan Yang, Shasha Xing, Fang Bian, Wanjing Yao, Xiangli Bai, Tao Zheng, Guangjie Wu, Si Jin

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Oxidative medicine and cellular longevity, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed, 1 pooled it
1.3field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed, 1 synthesis or guideline pooled it, 65 citations in OpenAlex.

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  17. The Key Role of Mitochondrial Function in Health and Disease.Antioxidants (Basel, Switzerland) · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Wenjing LiDepartment of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China ; The Key Laboratory of Drug Target Research and Pharmacodynamic Evaluation of Hubei Province, Wuhan 430030, China.
Xiaoyan YangDepartment of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China ; The Key Laboratory of Drug Target Research and Pharmacodynamic Evaluation of Hubei Province, Wuhan 430030, China.
Shasha XingDepartment of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China ; The Key Laboratory of Drug Target Research and Pharmacodynamic Evaluation of Hubei Province, Wuhan 430030, China.
Fang BianDepartment of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China ; The Key Laboratory of Drug Target Research and Pharmacodynamic Evaluation of Hubei Province, Wuhan 430030, China.
Wanjing YaoDepartment of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China ; The Key Laboratory of Drug Target Research and Pharmacodynamic Evaluation of Hubei Province, Wuhan 430030, China.
Xiangli BaiDepartment of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China ; The Key Laboratory of Drug Target Research and Pharmacodynamic Evaluation of Hubei Province, Wuhan 430030, China.
Tao ZhengDepartment of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China ; The Key Laboratory of Drug Target Research and Pharmacodynamic Evaluation of Hubei Province, Wuhan 430030, China.ORCID 0000-0003-1742-1357
Guangjie WuDepartment of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China ; The Key Laboratory of Drug Target Research and Pharmacodynamic Evaluation of Hubei Province, Wuhan 430030, China.
Si JinDepartment of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China ; The Key Laboratory of Drug Target Research and Pharmacodynamic Evaluation of Hubei Province, Wuhan 430030, China.
Huazhong University of Science and Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Oxidized low density of lipoprotein (oxLDL) is the major lipid found in atherosclerotic lesion and elevated plasma oxLDL is recognized to be a risk factor of atherosclerosis. Whether plasma oxLDL could be transported across endothelial cells and initiate atherosclerotic changes remains unknown. In an established in vitro cellular transcytosis model, the present study found that oxLDL could traffic across vascular endothelial cells and further that the regulation of endogenous ceramide production by ceramide metabolizing enzyme inhibitors significantly altered the transcytosis of oxLDL across endothelial cells. It was found that acid sphingomyelinase inhibitor, desipramine (DES), and de novo ceramide synthesis inhibitor, myriocin (MYR), both decreasing the endogenous ceramide production, significantly inhibited the transcytosis of oxLDL. Ceramidase inhibitor, N-oleoylethanolamine (NOE), and sphingomyelin synthase inhibitor, O-Tricyclo[5.2.1.02,6]dec-9-yl dithiocarbonate potassium salt (D609), both increasing the endogenous ceramide production, significantly upregulated the transcytosis of oxLDL. In vivo, injection of fluorescence labeled oxLDL into mice body also predisposed to the subendothelial retention of these oxidized lipids. The observations provided in the present study demonstrate that endogenous ceramide contributes to the transcytosis of oxLDL across endothelial cells and promotes the initiating step of atherosclerosis-the subendothelial retention of lipids in vascular wall.

Indexed as

AnimalsBridged-Ring CompoundsCeramidasesCeramidesDesipramineEndocannabinoidsEndothelial CellsEthanolaminesFatty Acids, MonounsaturatedHumansHuman Umbilical Vein Endothelial CellsLipoproteins, LDLMiceMice, Inbred C57BLNorbornanesOleic AcidsBridged-Ring CompoundsCeramidasesCeramidesDesipramineEndocannabinoidsEthanolaminesFatty Acids, MonounsaturatedLipoproteins, LDLN-oleoylethanolamineNorbornanesOleic Acidsoxidized low density lipoproteinphosphatidylcholine-ceramide phosphocholine transferaseSphingomyelin PhosphodiesteraseSphingosine N-AcyltransferasethermozymocidinThiocarbamatesThionesTransferases (Other Substituted Phosphate Groups)tricyclodecane-9-yl-xanthogenate

Identifiers

PMID24817993
PMCPMC4003761
OpenAlexW2030212361

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.