ArticleFrontiers in plant science2014
Monitoring protein turnover during phosphate starvation-dependent autophagic degradation using a photoconvertible fluorescent protein aggregate in tobacco BY-2 cells.
Article in Frontiers in plant science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- Autophagy maintains endosperm quality during seed storage to preserve germination ability in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Role of autophagy-related proteins ATG8f and ATG8h in the maintenance of autophagic activity inFrontiers in plant science · 2023Article
- Review
- Defects in autophagy lead to selective in vivo changes in turnover of cytosolic and organelle proteins in Arabidopsis.The Plant cell · 2022Article
- Intracellular phosphate recycling systems for survival during phosphate starvation in plants.Frontiers in plant science · 2022Review
- RCB-mediated chlorophagy caused by oversupply of nitrogen suppresses phosphate-starvation stress in plants.Plant physiology · 2021Article
- Autophagy mediates grain yield and nitrogen stress resistance by modulating nitrogen remobilization in rice.PloS one · 2021Article
- Cell Cycle-Dependence of Autophagic Activity and Inhibition of Autophagosome Formation at M Phase in Tobacco BY-2 Cells.International journal of molecular sciences · 2020Article
- The Local Phosphate Deficiency Response Activates Endoplasmic Reticulum Stress-Dependent Autophagy.Plant physiology · 2019Article
- Applications of phototransformable fluorescent proteins for tracking the dynamics of cellular components.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2015Review
- Differentiating phosphate-dependent and phosphate-independent systemic phosphate-starvation response networks in Arabidopsis thaliana through the application of phosphite.Journal of experimental botany · 2015Article
- Dissection of autophagy in tobacco BY-2 cells under sucrose starvation conditions using the vacuolar H(+)-ATPase inhibitor concanamycin A and the autophagy-related protein Atg8.Plant signaling & behavior · 2015Article
- Roles of autophagy in male reproductive development in plants.Frontiers in plant science · 2014Review
- Autophagy in plants and algae.Frontiers in plant science · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a system for quantitative monitoring of autophagic degradation in transformed tobacco BY-2 cells using an aggregate-prone protein comprised of cytochrome b5 (Cyt b5) and a tetrameric red fluorescent protein (RFP). Unfortunately, this system is of limited use for monitoring the kinetics of autophagic degradation because the proteins synthesized before and after induction of autophagy cannot be distinguished. To overcome this problem, we developed a system using kikume green-red (KikGR), a photoconvertible and tetrameric fluorescent protein that changes its fluorescence from green to red upon irradiation with purple light. Using the fusion protein of Cyt b5 and KikGR together with a method for the bulk conversion of KikGR, which we had previously used to convert the Golgi-localized monomeric KikGR fusion protein, we were able to monitor both the growth and de novo formation of aggregates. Using this system, we found that tobacco cells do not cease protein synthesis under conditions of phosphate (Pi)-starvation. Induction of autophagy under Pi-starvation, but not under sugar- or nitrogen-starvation, was specifically inhibited by phosphite, which is an analog of Pi with a different oxidation number. Therefore, the mechanism by which BY-2 cells can sense Pi-starvation and induce autophagy does not involve sensing a general decrease in energy supply and a specific Pi sensor might be involved in the induction of autophagy under Pi-starvation.
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