ArticleAnnals of botany2014
Dinitrogen fixation associated with shoots of aquatic carnivorous plants: is it ecologically important?
Article in Annals of botany, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 19 citations in OpenAlex.
- Construction costs and tradeoffs in carnivorous pitcher plant leaves: towards a pitcher leaf economics spectrum.Annals of botany · 2025Review
- Microbial community dynamics in two Central European peatlands affected by different nitrogen depositions.FEMS microbiology ecology · 2025Article
- To eat or not to eat: novel stable isotope models reveal a shift in carnivory with nutrient availability for aquatic Utricularia spp.Annals of botany · 2024Article
- CarnivorousApplied and environmental microbiology · 2023Article
- Hunters or farmers? Microbiome characteristics help elucidate the diet composition in an aquatic carnivorous plant.Microbiome · 2018Article
- Occurrence of myo-inositol and alkyl-substituted polysaccharide in the prey-trapping mucilage of Drosera capensis.Die Naturwissenschaften · 2017Article
- The Metagenome of Utricularia gibba's Traps: Into the Microbial Input to a Carnivorous Plant.PloS one · 2016Article
- The transcriptome of Utricularia vulgaris, a rootless plant with minimalist genome, reveals extreme alternative splicing and only moderate sequence similarity with Utricularia gibba.BMC plant biology · 2015Article
- Capture of algae promotes growth and propagation in aquatic Utricularia.Annals of botany · 2015Article
- Metatranscriptome analysis reveals host-microbiome interactions in traps of carnivorous Genlisea species.Frontiers in microbiology · 2015Article
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Authors and funding
11 authors at 7 institutions in 3 countries.
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No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsRootless carnivorous plants of the genus Utricularia are important components of many standing waters worldwide, as well as suitable model organisms for studying plant-microbe interactions. In this study, an investigation was made of the importance of microbial dinitrogen (N2) fixation in the N acquisition of four aquatic Utricularia species and another aquatic carnivorous plant, Aldrovanda vesiculosa.
methods16S rRNA amplicon sequencing was used to assess the presence of micro-organisms with known ability to fix N2. Next-generation sequencing provided information on the expression of N2 fixation-associated genes. N2 fixation rates were measured following (15)N2-labelling and were used to calculate the plant assimilation rate of microbially fixed N2. KEY
resultsUtricularia traps were confirmed as primary sites of N2 fixation, with up to 16 % of the plant-associated microbial community consisting of bacteria capable of fixing N2. Of these, rhizobia were the most abundant group. Nitrogen fixation rates increased with increasing shoot age, but never exceeded 1·3 μmol N g(-1) d. mass d(-1). Plant assimilation rates of fixed N2 were detectable and significant, but this fraction formed less than 1 % of daily plant N gain. Although trap fluid provides conditions favourable for microbial N2 fixation, levels of nif gene transcription comprised <0·01 % of the total prokaryotic transcripts.
conclusionsIt is hypothesized that the reason for limited N2 fixation in aquatic Utricularia, despite the large potential capacity, is the high concentration of NH4-N (2·0-4·3 mg L(-1)) in the trap fluid. Resulting from fast turnover of organic detritus, it probably inhibits N2 fixation in most of the microorganisms present. Nitrogen fixation is not expected to contribute significantly to N nutrition of aquatic carnivorous plants under their typical growth conditions; however, on an annual basis the plant-microbe system can supply nitrogen in the order of hundreds of mg m(-2) into the nutrient-limited littoral zone, where it may thus represent an important N source.
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