ArticleMolecular biology reports2014
Bioinformatics study of delta-12 fatty acid desaturase 2 (FAD2) gene in oilseeds.
Article in Molecular biology reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 67 citations in OpenAlex.
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- Reassessing fatty acid divergence: climatic and geographic constraints on origin traceability in global oil crops.NPJ science of food · 2025Article
- Volatile aromatic substances analysis of different temperature stored 'Docteur Jules Guyot' pear (Frontiers in plant science · 2025Article
- Characterization of fatty acid desaturase gene family inFrontiers in plant science · 2025Article
- Homologous Delta-12 Fatty Acid Desaturase (Journal of fungi (Basel, Switzerland) · 2024Article
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- Transcriptome Identification and Analysis of Fatty Acid Desaturase Gene Expression at Different Temperatures inMicroorganisms · 2023Article
- Genome-Wide Characterization of TomatoPlants (Basel, Switzerland) · 2023Article
- Anemochorous and zoochorous seeds of trees from the Brazilian savannas differ in fatty acid content and composition.AoB PLANTS · 2023Article
- Mapping of quantitative trait loci for the nutritional value of fresh market tomato.Functional & integrative genomics · 2023Article
- Identification of high seed oil yield and high oleic acid content in Brazilian germplasm of winter squash (Saudi journal of biological sciences · 2022Article
- Comparative Analysis and Structural Modeling ofBiology · 2022Article
- SNP discovery and structural insights into OeFAD2 unravelling high oleic/linoleic ratio in olive oil.Computational and structural biotechnology journal · 2022Article
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- In Silico Analysis of Partial Fatty Acid Desaturase 2 cDNA FromBioinformatics and biology insights · 2021Article
- Abscisic Acid Improves Linoleic Acid Accumulation Possibly by Promoting Expression ofFrontiers in plant science · 2021Article
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- Integration of miRNAs, Degradome, and Transcriptome Omics Uncovers a Complex Regulatory Network and Provides Insights Into Lipid and Fatty Acid Synthesis During Sesame Seed Development.Frontiers in plant science · 2021Article
- Preharvest UV-C Hormesis Induces Key Genes Associated With Homeostasis, Growth and Defense in Lettuce Inoculated WithFrontiers in plant science · 2021Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fatty acid desaturases constitute a group of enzymes that introduce double bonds into the hydrocarbon chains of fatty acids to produce unsaturated fatty acids. In plants, seed-specific delta-12 fatty acid desaturase 2 (FAD2) is responsible for the high content of linoleic acid by inserting a double bond at the delta-12 (omega-6) position of oleic acid. In this study, sixteen FAD2 and FAD2-2 protein sequences from oilseeds were analyzed by computational tools including two databases of the NCBI and EXPASY and data management tools such as SignalP, TMHMM, Psort, ProtParam, TargetP, PLACE and PlantCARE. These services were used to predict the protein properties such as molecular mass, pI, signal peptide, transmembrane and conserved domains, secondary and spatial structures. The polypeptide sequences were aligned and a neighbour-joining tree was constructed using MEGA5.1 to elucidate phylogenetic relationships among FAD2 genes. Based on the phylogenetic analysis species with high similarity in FAD2 sequence grouped together. FAD2 proteins include highly conserved histidine-rich motifs (HECGHH, HRRHH and HV[A/C/T]HH) that are located by three to five transmembrane anchors. For further investigations Sesamum indicum FAD2 was selected and analyzed by bioinformatics tools. Analysis showed no N-terminal signal peptide for probable localization of FAD2 protein in cytoplasmic organelles such as chloroplast, mitochondria and Golgi. Instead the C-terminal signaling motif YNNKL, Y(K/N)NKF or YRNKI allows FAD2 protein to selectively bind to and embed in the endoplasmic reticulum. FAD2 promoter contains different cis-regulatory elements involve in the biotic and abiotic stresses response or control of gene expression specifically in seeds.
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