ArticlePloS one2014
Integration of molecular profiling and chemical imaging to elucidate fibroblast-microenvironment impact on cancer cell phenotype and endocrine resistance in breast cancer.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 38 citations in OpenAlex.
- Cell Phase Identification in a Three-Dimensional Engineered Tumor Model by Infrared Spectroscopic Imaging.Analytical chemistry · 2023Article
- Extracellular Matrix-Bound FGF2 Mediates Estrogen Receptor Signaling and Therapeutic Response in Breast Cancer.Molecular cancer research : MCR · 2021Article
- A review of the endocrine resistance in hormone-positive breast cancer.American journal of cancer research · 2021Review
- Intrinsic and Extrinsic Factors Governing the Transcriptional Regulation of ESR1.Hormones & cancer · 2020Review
- Infrared spectral microscopy as a tool to monitor lung fibrosis development in a model system.Biomedical optics express · 2020Article
- An Innovative Platform Merging Elemental Analysis and Ftir Imaging for Breast Tissue Analysis.Scientific reports · 2019Article
- Application of Vibrational Spectroscopy and Imaging to Point-of-Care Medicine: A Review.Applied spectroscopy · 2018Review
- Simultaneous cancer and tumor microenvironment subtyping using confocal infrared microscopy for all-digital molecular histopathology.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Targeting the tumour stroma to improve cancer therapy.Nature reviews. Clinical oncology · 2018Review
- Infrared Spectroscopic Imaging Advances as an Analytical Technology for Biomedical Sciences.Analytical chemistry · 2018Review
- Infrared spectroscopic imaging: Label-free biochemical analysis of stroma and tissue fibrosis.The international journal of biochemistry & cell biology · 2017Review
- Structurally Novel Antiestrogens Elicit Differential Responses from Constitutively Active Mutant Estrogen Receptors in Breast Cancer Cells and Tumors.Cancer research · 2017Article
- Insights into Biochemical Alteration in Cancer-Associated Fibroblasts by using Novel Correlative Spectroscopy.ChemistryOpen · 2017Article
- Proteins regulating the intercellular transfer and function of P-glycoprotein in multidrug-resistant cancer.Ecancermedicalscience · 2017Review
- Characterization of the Tumor Microenvironment and Tumor-Stroma Interaction by Non-invasive Preclinical Imaging.Frontiers in oncology · 2017Review
- Emerging Themes in Image Informatics and Molecular Analysis for Digital Pathology.Annual review of biomedical engineering · 2016Review
- Letrozole-induced functional changes in carcinoma-associated fibroblasts and their influence on breast cancer cell biology.Medical oncology (Northwood, London, England) · 2016Article
- Development of a practical spatial-spectral analysis protocol for breast histopathology using Fourier transform infrared spectroscopic imaging.Faraday discussions · 2016Article
- Microenvironment and endocrine resistance in breast cancer: Friend or foe?World journal of clinical oncology · 2015Article
- Raman spectroscopic sensing of carbonate intercalation in breast microcalcifications at stereotactic biopsy.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The tumor microenvironment is known to play a key role in altering the properties and behavior of nearby cancer cells. Its influence on resistance to endocrine therapy and cancer relapse, however, is poorly understood. Here we investigate the interaction of mammary fibroblasts and estrogen receptor-positive breast cancer cells in three-dimensional culture models in order to characterize gene expression, cellular changes, and the secreted protein factors involved in the cellular cross-talk. We show that fibroblasts, which are the predominant cell type found in the stroma adjacent to the cancer cells in a tumor, induce an epithelial-to-mesenchymal transition in the cancer cells, leading to hormone-independent growth, a more invasive phenotype, and resistance to endocrine therapy. Here, we applied a label-free chemical imaging modality, Fourier transform infrared (FT-IR) spectroscopic imaging, to identify cells that had transitioned to hormone-independent growth. Both the molecular and chemical profiles identified here were translated from cell culture to patient samples: a secreted protein signature was used to stratify patient populations based on gene expression and FT-IR was used to characterize breast tumor patient biopsies. Our findings underscore the role of mammary fibroblasts in promoting aggressiveness and endocrine therapy resistance in ER-positive breast cancers and highlight the utility of FT-IR for the further characterization of breast cancer samples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.