ArticleJournal of bacteriology2014
Genetic dissection of the mamAB and mms6 operons reveals a gene set essential for magnetosome biogenesis in Magnetospirillum gryphiswaldense.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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58 citing papers in PubMed, 124 citations in OpenAlex.
- A cumate-inducible promoter system for tuned gene expression and magnetosome overproduction in magnetotactic bacteria.Applied microbiology and biotechnology · 2026Article
- Cross-linking proteomics analysis for reliable identification of MamXY-associated magnetosome formation.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Biosynthesis and function of magnetic organelles in magnetotactic bacteria.Nature reviews. Microbiology · 2026Review
- The Role of Confinement in Biomineralization.Chemical reviews · 2025Review
- Phylogenetic and genomic insights into magnetosome biomineralization in magnetotacticApplied and environmental microbiology · 2025Article
- A network of coiled-coil and actin-like proteins controls the cellular organization of magnetosome organelles in deep-branching magnetotactic bacteria.Nature communications · 2025Article
- Magnetizing Biotech-Advances in (In Vivo) Magnetic Enzyme Immobilization.Engineering in life sciences · 2025Review
- Magnetochrome-catalyzed oxidation of ferrous iron by MamP enables magnetite crystal growth in the magnetotactic bacterium AMB-1.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- MamF-like proteins are distant Tic20 homologs involved in organelle assembly in bacteria.Nature communications · 2024Article
- Bacterial Organelles in Iron Physiology.Molecular microbiology · 2024Review
- Essential magnetosome proteins MamI and MamL from magnetotactic bacteria interact in mammalian cells.Scientific reports · 2024Article
- Diversity and structural-functional insights of alpha-solenoid proteins.Protein science : a publication of the Protein Society · 2024Review
- Understanding microbial biomineralization at the molecular level: recent advances.World journal of microbiology & biotechnology · 2024Review
- Intrinsic and extrinsic determinants of conditional localization of Mms6 to magnetosome organelles inJournal of bacteriology · 2024Article
- Nitric oxide sensor NsrR is the key direct regulator of magnetosome formation and nitrogen metabolism in Magnetospirillum.Nucleic acids research · 2024Article
- Article
- Comparing the Colloidal Stabilities of Commercial and Biogenic Iron Oxide Nanoparticles That Have Potential In Vitro/In Vivo Applications.Molecules (Basel, Switzerland) · 2023Article
- Silent gene clusters encode magnetic organelle biosynthesis in a non-magnetotactic phototrophic bacterium.The ISME journal · 2023Article
- Key gene networks that control magnetosome biomineralization in magnetotactic bacteria.National science review · 2023Article
- The transcriptomic landscape of Magnetospirillum gryphiswaldense during magnetosome biomineralization.BMC genomics · 2022Article
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biosynthesis of bacterial magnetosomes, which are intracellular membrane-enclosed, nanosized magnetic crystals, is controlled by a set of >30 specific genes. In Magnetospirillum gryphiswaldense, these are clustered mostly within a large conserved genomic magnetosome island (MAI) comprising the mms6, mamGFDC, mamAB, and mamXY operons. Here, we demonstrate that the five previously uncharacterized genes of the mms6 operon have crucial functions in the regulation of magnetosome biomineralization that partially overlap MamF and other proteins encoded by the adjacent mamGFDC operon. While all other deletions resulted in size reduction, elimination of either mms36 or mms48 caused the synthesis of magnetite crystals larger than those in the wild type (WT). Whereas the mms6 operon encodes accessory factors for crystal maturation, the large mamAB operon contains several essential and nonessential genes involved in various other steps of magnetosome biosynthesis, as shown by single deletions of all mamAB genes. While single deletions of mamL, -P, -Q, -R, -B, -S, -T, and -U showed phenotypes similar to those of their orthologs in a previous study in the related M. magneticum, we found mamI and mamN to be not required for at least rudimentary iron biomineralization in M. gryphiswaldense. Thus, only mamE, -L, -M, -O, -Q, and -B were essential for formation of magnetite, whereas a mamI mutant still biomineralized tiny particles which, however, consisted of the nonmagnetic iron oxide hematite, as shown by high-resolution transmission electron microscopy (HRTEM) and the X-ray absorption near-edge structure (XANES). Based on this and previous studies, we propose an extended model for magnetosome biosynthesis in M. gryphiswaldense.
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