ArticleJournal of bacteriology2014
Tolerance of a phage element by Streptococcus pneumoniae leads to a fitness defect during colonization.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed, 26 citations in OpenAlex.
- WaaL-WaaP receptor cross-talk drives phage-induced collateral sensitivity to colistin in Salmonella Pullorum.Veterinary research · 2026Article
- Prophages in the infant gut are pervasively induced and may modulate the functionality of their hosts.NPJ biofilms and microbiomes · 2025Article
- Wild-typeFrontiers in microbiology · 2023Article
- Pneumococcal within-host diversity during colonization, transmission and treatment.Nature microbiology · 2022Article
- The effectiveness of extended binding affinity of prophage lysin PlyARI against Streptococcus suis infection.Archives of microbiology · 2021Article
- Lysogeny inMicroorganisms · 2020Review
- Interactions of Bacteriophages and Bacteria at the Airway Mucosa: New Insights Into the Pathophysiology of Asthma.Frontiers in allergy · 2020Review
- Interactions between Bacteriophages and Eukaryotic Cells.Scientifica · 2020Review
- Methylation Warfare: Interaction of Pneumococcal Bacteriophages with Their Host.Journal of bacteriology · 2019Article
- Characterization of Bacteria and Inducible Phages in an Intensive Care Unit.Journal of clinical medicine · 2019Article
- Pneumococcal quorum sensing drives an asymmetric owner-intruder competitive strategy during carriage via the competence regulon.Nature microbiology · 2019Article
- Global emergence and population dynamics of divergent serotype 3 CC180 pneumococci.PLoS pathogens · 2018Article
- Capsule Prolongs Survival of Streptococcus pneumoniae during Starvation.Infection and immunity · 2018Article
- Characterization of a Prophage-Free Derivative Strain ofFrontiers in microbiology · 2018Article
- Puzzling Over the Pneumococcal Pangenome.Frontiers in microbiology · 2018Review
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- Infant Mouse Model for the Study of Shedding and Transmission during Streptococcus pneumoniae Monoinfection.Infection and immunity · 2016Article
- Friendly Fire: Biological Functions and Consequences of Chromosomal Targeting by CRISPR-Cas Systems.Journal of bacteriology · 2016Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The pathogenesis of the disease caused by Streptococcus pneumoniae begins with colonization of the upper respiratory tract. Temperate phages have been identified in the genomes of up to 70% of clinical isolates. How these phages affect the bacterial host during colonization is unknown. Here, we examined a clinical isolate that carries a novel prophage element, designated Spn1, which was detected in both integrated and episomal forms. Surprisingly, both lytic and lysogenic Spn1 genes were expressed under routine growth conditions. Using a mouse model of asymptomatic colonization, we demonstrate that the Spn1(-) strain outcompeted the Spn1(+) strain >70-fold. To determine if Spn1 causes a fitness defect through a trans-acting factor, we constructed an Spn1(+) mutant that does not become an episome or express phage genes. This mutant competed equally with the Spn1(-) strain, indicating that expression of phage genes or phage lytic activity is required to confer this fitness defect. In vitro, we demonstrate that the presence of Spn1 correlated with a defect in LytA-mediated autolysis. Furthermore, the Spn1(+) strain displayed increased chain length and resistance to lysis by penicillin compared to the Spn(-) strain, indicating that Spn1 alters the cell wall physiology of its host strain. We posit that these changes in cell wall physiology allow for tolerance of phage gene products and are responsible for the relative defect of the Spn1(+) strain during colonization. This study provides new insight into how bacteria and prophages interact and affect bacterial fitness in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.