ArticlePloS one2014
Development and validation of a real-time PCR for Chlamydia suis diagnosis in swine and humans.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 36 citations in OpenAlex.
- Prevalence and Tetracycline Susceptibility ofMicroorganisms · 2026Article
- Small intestinal organoids as a model to study interactions ofFrontiers in microbiology · 2026Article
- Seroprevalence and Risk Factors ofVector borne and zoonotic diseases (Larchmont, N.Y.) · 2024Article
- Zoonotic and other veterinary chlamydiae - an update, the role of the plasmid and plasmid-mediated transformation.Pathogens and disease · 2024Review
- Gut microbiome diversity of porcine peritonitis model of sepsis.Scientific reports · 2022Article
- Environment and Offspring Surveillance in Porcine Brucellosis.Frontiers in veterinary science · 2022Article
- Transferrins Reduce Replication ofPathogens (Basel, Switzerland) · 2021Article
- Generation of Tetracycline and Rifamycin ResistantFrontiers in microbiology · 2021Article
- Analysis of the vaginal microbiome of giant pandas using metagenomics sequencing.MicrobiologyOpen · 2020Article
- Establishment of a Multiplex Real-Time TaqMan-MGB Polymerase Chain Reaction (PCR) Method for the Simultaneous Detection of Three Animal Chlamydia Species.Medical science monitor : international medical journal of experimental and clinical research · 2019Article
- Antimicrobial Resistance inMicrobiology spectrum · 2018Article
- Asymptomatic infections with highly polymorphic Chlamydia suis are ubiquitous in pigs.BMC veterinary research · 2017Article
- Chlamydia prevalence in Polish pig herds.Epidemiology and infection · 2016Article
- Article
- Evaluation of the presence and zoonotic transmission of Chlamydia suis in a pig slaughterhouse.BMC infectious diseases · 2014Article
- Chlamydial Antibiotic Resistance and Treatment Failure in Veterinary and Human Medicine.Current clinical microbiology reportsReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pigs are the natural host for Chlamydia suis, a pathogen which is phylogenetically highly related to the human pathogen C. trachomatis. Chlamydia suis infections are generally treated with tetracyclines. In 1998, tetracyline resistant C. suis strains emerged on U.S. pig farms and they are currently present in the Belgian, Cypriote, German, Israeli, Italian and Swiss pig industry. Infections with tetracycline resistant C. suis strains are mainly associated with severe reproductive failure leading to marked economical loss. We developed a sensitive and specific TaqMan probe-based C. suis real-time PCR for examining clinical samples of both pigs and humans. The analytical sensitivity of the real-time PCR is 10 rDNA copies/reaction without cross-amplifying DNA of other Chlamydia species. The PCR was successfully validated using conjunctival, pharyngeal and stool samples of slaughterhouse employees, as well as porcine samples from two farms with evidence of reproductive failure and one farm without clinical disease. Chlamydia suis was only detected in diseased pigs and in the eyes of humans. Positive humans had no clinical complaints. PCR results were confirmed by culture in McCoy cells. In addition, Chlamydia suis isolates were also examined by the tet(C) PCR, designed for demonstrating the tetracycline resistance gene tet(C). The tet(C) gene was only present in porcine C. suis isolates.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.