ArticleStem cell research2014
Three dimensional cellular microarray platform for human neural stem cell differentiation and toxicology.
Article in Stem cell research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 61 citations in OpenAlex.
- Comprehensive characterization of the human neural stem cell line HNSC.100 as a versatile model for neurobiological research.Biochemistry and biophysics reports · 2026Article
- Cell-embedded microgels as emerging miniature 3D tissue-mimics toward biochip-based toxicity screening.Bioengineering & translational medicine · 2026Review
- Directional Submicrofiber Hydrogel Composite Scaffolds Supporting Neuron Differentiation and Enabling Neurite Alignment.International journal of molecular sciences · 2022Article
- Tuning Physicochemical Properties of a Macroporous Polysaccharide-Based Scaffold for 3D Neuronal Culture.International journal of molecular sciences · 2021Article
- Article
- Stem cells technology: a powerful tool behind new brain treatments.Drug delivery and translational research · 2018Review
- A Novel Cell Penetrating Peptide for the Differentiation of Human Neural Stem Cells.Biomolecules · 2018Article
- A 3D bioinspired highly porous polymeric scaffolding system forRSC advances · 2018Article
- Spatially and Temporally Controlled Hydrogels for Tissue Engineering.Materials science & engineering. R, Reports : a review journal · 2017Article
- NanoEHS beyond Toxicity - Focusing on Biocorona.Environmental science. Nano · 2017Article
- Nanopatterned Extracellular Matrices Enable Cell-Based Assays with a Mass Spectrometric Readout.Nano letters · 2017Article
- High-Throughput Toxicity and Phenotypic Screening of 3D Human Neural Progenitor Cell Cultures on a Microarray Chip Platform.Stem cell reports · 2016Article
- Living Cell Microarrays: An Overview of Concepts.Microarrays (Basel, Switzerland) · 2016Review
- State-of-the-Art Metabolic Toxicity Screening and Pathway Evaluation.Analytical chemistry · 2016Article
- Human proximal tubule cells form functional microtissues.Pflugers Archiv : European journal of physiology · 2016Article
- Cell-Based Assay Design for High-Content Screening of Drug Candidates.Journal of microbiology and biotechnology · 2016Review
- Transplantation of mouse embryonic stem cell-derived oligodendrocytes in the murine model of globoid cell leukodystrophy.Stem cell research & therapy · 2015Article
- Prospects of Natural Polymeric Scaffolds in Peripheral Nerve Tissue-Regeneration.Advances in experimental medicine and biologyReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
We developed a three-dimensional (3D) cellular microarray platform for the high-throughput (HT) analysis of human neural stem cell (hNSC) growth and differentiation. The growth of an immortalized hNSC line, ReNcell VM, was evaluated on a miniaturized cell culture chip consisting of 60nl spots of cells encapsulated in alginate, and compared to standard 2D well plate culture conditions. Using a live/dead cell viability assay, we demonstrated that the hNSCs are able to expand on-chip, albeit with lower proliferation rates and viabilities than in conventional 2D culture platforms. Using an in-cell, on-chip immunofluorescence assay, which provides quantitative information on cellular levels of proteins involved in neural fate, we demonstrated that ReNcell VM can preserve its multipotent state during on-chip expansion. Moreover, differentiation of the hNSCs into glial progeny was achieved both off- and on-chip six days after growth factor removal, accompanied by a decrease in the neural progenitor markers. The versatility of the platform was further demonstrated by complementing the cell culture chip with a chamber system that allowed us to screen for differential toxicity of small molecules to hNSCs. Using this approach, we showed differential toxicity when evaluating three neurotoxic compounds and one antiproliferative compound, and the null effect of a non-toxic compound at relevant concentrations. Thus, our 3D high-throughput microarray platform may help predict, in vitro, which compounds pose an increased threat to neural development and should therefore be prioritized for further screening and evaluation.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.