ArticleChemistry & biology2014
In vivo photoswitchable flow cytometry for direct tracking of single circulating tumor cells.
Article in Chemistry & biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- Targeted Single-cell Isolation of Spontaneously Escaping Live Melanoma Cells for Comparative Transcriptomics.Cancer research communications · 2023Article
- Current methods for studying metastatic potential of tumor cells.Cancer cell international · 2022Review
- Nanoplateletsomes restrain metastatic tumor formation through decoy and active targeting in a preclinical mouse model.Acta pharmaceutica Sinica. B · 2022Article
- Measuring kinetics and metastatic propensity of CTCs by blood exchange between mice.Nature communications · 2021Article
- Article
- Time-gated luminescence imaging for background free in vivo tracking of single circulating tumor cells.Optics letters · 2020Article
- Heterogeneity of circulating tumor cell dissemination and lung metastases in a subcutaneous Lewis lung carcinoma model.Biomedical optics express · 2020Article
- Frontiers in Intravital Multiphoton Microscopy of Cancer.Cancer reports (Hoboken, N.J.) · 2020Review
- Prospects for the Use of Upconverting Nanoparticles as a Contrast Agent for Enumeration of Circulating Cells in vivo.International journal of nanomedicine · 2020Article
- Photoswitchable Spasers with a Plasmonic Core and Photoswitchable Fluorescent Proteins.Scientific reports · 2019Article
- Detection of Apoptotic Circulating Tumor Cells Using in vivo Fluorescence Flow Cytometry.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2019Article
- Optofluidic real-time cell sorter for longitudinal CTC studies in mouse models of cancer.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Millisecond cellular labellingBiomedical optics express · 2018Article
- Fluorescence detection, enumeration and characterization of single circulating cells in vivo: technology, applications and future prospects.Physics in medicine and biology · 2017Article
- In vivo noninvasive analysis of graphene nanomaterial pharmacokinetics using photoacoustic flow cytometry.Journal of applied toxicology : JAT · 2017Article
- Real-time monitoring of circulating tumor cell (CTC) release after nanodrug or tumor radiotherapy using in vivo flow cytometry.Biochemical and biophysical research communications · 2017Article
- Microfluidic System for In-Flow Reversible Photoswitching of Near-Infrared Fluorescent Proteins.Analytical chemistry · 2016Article
- Photoswitchable non-fluorescent thermochromic dye-nanoparticle hybrid probes.Scientific reports · 2016Article
- Two-photon excited photoconversion of cyanine-based dyes.Scientific reports · 2016Article
- Multicolor multiphoton in vivo imaging flow cytometry.Optics express · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Photoswitchable fluorescent proteins (PSFPs) that change their color in response to light have led to breakthroughs in studying static cells. However, using PSFPs to study cells in dynamic conditions is challenging. Here we introduce a method for in vivo ultrafast photoswitching of PSFPs that provides labeling and tracking of single circulating cells. Using in vivo multicolor flow cytometry, this method demonstrated the capability for studying recirculation, migration, and distribution of circulating tumor cells (CTCs) during metastasis progression. In tumor-bearing mice, it enabled monitoring of real-time dynamics of CTCs released from primary tumor, identifying dormant cells, and imaging of CTCs colonizing a primary tumor (self-seeding) or existing metastasis (reseeding). Integration of genetically encoded PSFPs, fast photoswitching, flow cytometry, and imaging makes in vivo single cell analysis in the circulation feasible to provide insights into the behavior of CTCs and potentially immune-related and bacterial cells in circulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.