ArticleActa crystallographica. Section D, Biological crystallography2014
In cellulo structure determination of a novel cypovirus polyhedrin.
Article in Acta crystallographica. Section D, Biological crystallography, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Review
- Intracellular protein crystallization in living insect cells.FEBS open bio · 2025Article
- High-throughput structure determination of an intrinsically disordered protein using cell-free protein crystallization.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A streamlined approach to structure elucidation using in cellulo crystallized recombinant proteins, InCellCryst.Nature communications · 2024Article
- Atomic structure of a nudivirus occlusion body protein determined from a 70-year-old crystal sample.Nature communications · 2023Article
- ID23-2: an automated and high-performance microfocus beamline for macromolecular crystallography at the ESRF.Journal of synchrotron radiation · 2022Article
- Screening cells for crystals: a synergistic approach.Journal of applied crystallography · 2020Article
- Determination of Patterson group symmetry from sparse multi-crystal data sets in the presence of an indexing ambiguity.Acta crystallographica. Section D, Structural biology · 2018Article
- Towards in cellulo virus crystallography.Scientific reports · 2018Article
- Where is crystallography going?Acta crystallographica. Section D, Structural biology · 2018Review
- To be disordered or not to be disordered: is that still a question for proteins in the cell?Cellular and molecular life sciences : CMLS · 2017Review
- High-speed fixed-target serial virus crystallography.Nature methods · 2017Article
- Microcrystallography of Protein Crystals and In Cellulo Diffraction.Journal of visualized experiments : JoVE · 2017Article
- Low-dose fixed-target serial synchrotron crystallography.Acta crystallographica. Section D, Structural biology · 2017Article
- Current advances in synchrotron radiation instrumentation for MX experiments.Archives of biochemistry and biophysics · 2016Review
- A pipeline for structure determination of in vivo-grown crystals using in cellulo diffraction.Acta crystallographica. Section D, Structural biology · 2016Article
- Article
- An in cellulo-derived structure of PAK4 in complex with its inhibitor Inka1.Nature communications · 2015Article
- Polyhedra structures and the evolution of the insect viruses.Journal of structural biology · 2015Article
- In vivo protein crystallization in combination with highly brilliant radiation sources offers novel opportunities for the structural analysis of post-translationally modified eukaryotic proteins.Acta crystallographica. Section F, Structural biology communications · 2015Review
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4 authors.
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Abstract
This work demonstrates that with the use of a microfocus synchrotron beam the structure of a novel viral polyhedrin could be successfully determined from microcrystals within cells, removing the preparatory step of sample isolation and maintaining a favourable biological environment. The data obtained are of high quality, comparable to that obtained from isolated crystals, and enabled a facile structure determination. A small but significant difference is observed between the unit-cell parameters and the mosaic spread of in cellulo and isolated crystals, suggesting that even these robust crystals are adversely affected by removal from the cell.
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