ArticleActa crystallographica. Section D, Biological crystallography2014
Hitting the target: fragment screening with acoustic in situ co-crystallization of proteins plus fragment libraries on pin-mounted data-collection micromeshes.
Article in Acta crystallographica. Section D, Biological crystallography, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Lysozyme revisited: evaluating models of a reference protein in structural biology.Current research in structural biology · 2026Article
- Standard Sample Preparation for Serial Femtosecond Crystallography.Biomolecules · 2025Review
- Bragg Spot Finder (BSF): a new machine-learning-aided approach to deal with spot finding for rapidly filtering diffraction pattern images.Journal of applied crystallography · 2024Article
- Serial crystallography with multi-stage merging of thousands of images.Acta crystallographica. Section F, Structural biology communications · 2022Article
- A capillary-based microfluidic device enables primary high-throughput room-temperature crystallographic screening.Journal of applied crystallography · 2021Article
- FragMAXapp: crystallographic fragment-screening data-analysis and project-management system.Acta crystallographica. Section D, Structural biology · 2021Article
- Two Methods, One Goal: Structural Differences between Cocrystallization and Crystal Soaking to Discover Ligand Binding Poses.ChemMedChem · 2021Article
- Demonstration of the utility of DOS-derived fragment libraries for rapid hit derivatisation in a multidirectional fashion.Chemical science · 2020Article
- Rapid approach to complex boronic acids.Science advances · 2019Article
- On-chip crystallization for serial crystallography experiments and on-chip ligand-binding studies.IUCrJ · 2019Article
- Strategies for sample delivery for femtosecond crystallography.Acta crystallographica. Section D, Structural biology · 2019Review
- Miniaturization and optimization of 384-well compatible RNA sequencing library preparation.PloS one · 2019Article
- Using sound pulses to solve the crystal-harvesting bottleneck.Acta crystallographica. Section D, Structural biology · 2018Article
- Automated Protocols for Macromolecular Crystallization at the MRC Laboratory of Molecular Biology.Journal of visualized experiments : JoVE · 2018Article
- Gentle, fast and effective crystal soaking by acoustic dispensing.Acta crystallographica. Section D, Structural biology · 2017Article
- Ultrasonic acoustic levitation for fast frame rate X-ray protein crystallography at room temperature.Scientific reports · 2016Article
- Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography.Structure (London, England : 1993) · 2016Article
- Automated harvesting and processing of protein crystals through laser photoablation.Acta crystallographica. Section D, Structural biology · 2016Article
- In situ X-ray data collection and structure phasing of protein crystals at Structural Biology Center 19-ID.Journal of synchrotron radiation · 2015Article
- In meso in situ serial X-ray crystallography of soluble and membrane proteins.Acta crystallographica. Section D, Biological crystallography · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors.
Funding
Abstract
Acoustic droplet ejection (ADE) is a powerful technology that supports crystallographic applications such as growing, improving and manipulating protein crystals. A fragment-screening strategy is described that uses ADE to co-crystallize proteins with fragment libraries directly on MiTeGen MicroMeshes. Co-crystallization trials can be prepared rapidly and economically. The high speed of specimen preparation and the low consumption of fragment and protein allow the use of individual rather than pooled fragments. The Echo 550 liquid-handling instrument (Labcyte Inc., Sunnyvale, California, USA) generates droplets with accurate trajectories, which allows multiple co-crystallization experiments to be discretely positioned on a single data-collection micromesh. This accuracy also allows all components to be transferred through small apertures. Consequently, the crystallization tray is in equilibrium with the reservoir before, during and after the transfer of protein, precipitant and fragment to the micromesh on which crystallization will occur. This strict control of the specimen environment means that the crystallography experiments remain identical as the working volumes are decreased from the few microlitres level to the few nanolitres level. Using this system, lysozyme, thermolysin, trypsin and stachydrine demethylase crystals were co-crystallized with a small 33-compound mini-library to search for fragment hits. This technology pushes towards a much faster, more automated and more flexible strategy for structure-based drug discovery using as little as 2.5 nl of each major component.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.