ArticleBehavioural brain research2014
Sex differences in diazepam effects and parvalbumin-positive GABA neurons in trait anxiety Long Evans rats.
Article in Behavioural brain research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 37 citations in OpenAlex.
- Braking Parkinson's progression: the hypothetical druggable role of striatal parvalbumin interneurons.NPJ Parkinson's disease · 2026Review
- Possible GABAkine-Mediated Sedative-Like Antidepressant Effects of Phytol: Molecular Interventions Through In Vitro, In Vivo and In Silico Approaches.CNS neuroscience & therapeutics · 2025Article
- Prepubertal ovariectomy confers resilience to stress-induced anxiety in adult female mice.Psychoneuroendocrinology · 2023Article
- Parvalbumin Role in Epilepsy and Psychiatric Comorbidities: From Mechanism to Intervention.Frontiers in integrative neuroscience · 2022Review
- The Poly I:C maternal immune stimulation model shows unique patterns of brain metabolism, morphometry, and plasticity in female rats.Frontiers in behavioral neuroscience · 2022Article
- The Role of Parvalbumin Interneurons in Neurotransmitter Balance and Neurological Disease.Frontiers in psychiatry · 2021Review
- Regulation of Pv-specific interneurons in the medial prefrontal cortex and reward-seeking behaviors.Journal of neurochemistry · 2021Article
- PEN Receptor GPR83 in Anxiety-Like Behaviors: Differential Regulation in Global vs Amygdalar Knockdown.Frontiers in neuroscience · 2021Article
- Development and Molecular Investigation into the Effects of Carbamazepine Exposure in the Zebrafish (International journal of environmental research and public health · 2020Article
- Age-dependent sexual dimorphism in hippocampal cornu ammonis-1 perineuronal net expression in rats.Brain and behavior · 2019Article
- Sex matters: females in proestrus show greater diazepam anxiolysis and brain-derived neurotrophin factor- and parvalbumin-positive neurons than males.The European journal of neuroscience · 2018Article
- Sex and β-Endorphin Influence the Effects of Ethanol on LimbicFrontiers in genetics · 2018Article
- Parvalbumin Interneurons of Central Amygdala Regulate the Negative Affective States and the Expression of Corticotrophin-Releasing Hormone During Morphine Withdrawal.The international journal of neuropsychopharmacology · 2016Article
- Myristic acid produces anxiolytic-like effects in Wistar rats in the elevated plus maze.BioMed research international · 2014Article
- Lingual innervation in male and female marmosets.Neurobiology of pain (Cambridge, Mass.)Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
In clinical populations, prevalence rates for a number of anxiety disorders differ between males and females and gonadal hormones are thought to contribute to these differences. While these hormones have been shown to modulate the anxiolytic effects of the benzodiazepine agonist diazepam in some models, findings are inconsistent. Here, we tested for sex differences in response to anxiogenic stimuli following a 30-min diazepam (1.0mg/kg) pre-treatment in male and female rats showing high (HAn) and low (LAn) anxiety-like behavior on the elevated plus maze. Acute diazepam administration resulted in decreased anxiety-like behavior only in HAn males as demonstrated by a significant increase in percent open arm time in the elevated plus maze (EPM). Immunohistochemical analysis for parvalbumin (PV; a calcium-binding protein that selectively stains GABAergic neurons) in central amygdala (CeA), caudate putamen (CPu) and the hippocampus indicated the number of GABAergic interneurons in these areas differed across sex and anxiety trait. In the CPu, females had significantly more PV-immunoreactive (IR) cells than males, and LAn females had greater PV-IR neurons than HAn females. In the CeA, males displayed an increased number of PV-IR neurons compared to females, with no differences found between LAn and HAn. Further, trait differences were evident in the CA2 region of the hippocampus, regardless of sex. Taken together, these data suggest that gonadal hormones and trait anxiety may influence the sensitivity to the anti-anxiety effects of diazepam and these differences may be due in part to the distribution of GABA-containing interneurons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.