ArticleApplied and environmental microbiology2014
Discovery of bovine digital dermatitis-associated Treponema spp. in the dairy herd environment by a targeted deep-sequencing approach.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed, 79 citations in OpenAlex.
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- Real-time digital dermatitis detection in dairy cows on Android and iOS apps using computer vision techniques.Translational animal science · 2025Article
- Is Camphor the Future in Supporting Therapy for Skin Infections?Pharmaceuticals (Basel, Switzerland) · 2024Review
- Molecular Screening for Digital Dermatitis-Associated Treponemes in Bovine Ischaemic Teat Necrosis Lesions and Milk in Dairy Cattle.Pathogens (Basel, Switzerland) · 2024Article
- The Risk Factors of Dewclaw Dermatitis in Beef Cattle in the Amazon Biome.Animals : an open access journal from MDPI · 2024Article
- Correlation of lesion severity with bacterial changes in Treponeme-Associated Hoof Disease from free-roaming wild elk (Cervus canadensis).Animal microbiome · 2024Article
- Proinflammatory CD14Infection and immunity · 2024Article
- Effect of Antimicrobial Use in Conventional Versus Natural Cattle Feedlots on the Microbiome and Resistome.Microorganisms · 2023Article
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- Detection ofVeterinary research forum : an international quarterly journal · 2022Article
- Characterization of rumen, fecal, and milk microbiota in lactating dairy cows.Frontiers in microbiology · 2022Article
- The Presence ofMicroorganisms · 2021Article
- Relationships among bedding materials, bedding bacterial composition and lameness in dairy cows.Animal bioscience · 2021Article
- The dysbiosis of ovine foot microbiome during the development and treatment of contagious ovine digital dermatitis.Animal microbiome · 2021Article
- Detection of treponemes in digital dermatitis lesions of captive European bison (Bison bonasus).PloS one · 2021Article
- Microbiome Shift, Diversity, and Overabundance of Opportunistic Pathogens in Bovine Digital Dermatitis Revealed by 16S rRNA Amplicon Sequencing.Animals : an open access journal from MDPI · 2020Article
- Genotypic and Phenotypic Characterization ofMicroorganisms · 2020Article
- First cases of contagious ovine digital dermatitis in Germany.Acta veterinaria Scandinavica · 2020Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The bacteria associated with the infectious claw disease bovine digital dermatitis (DD) are spirochetes of the genus Treponema; however, their environmental reservoir remains unknown. To our knowledge, the current study is the first report of the discovery and phylogenetic characterization of rRNA gene sequences from DD-associated treponemes in the dairy herd environment. Although the spread of DD appears to be facilitated by wet floors covered with slurry, no DD-associated treponemes have been isolated from this environment previously. Consequently, there is a lack of knowledge about the spread of this disease among cows within a herd as well as between herds. To address the issue of DD infection reservoirs, we searched for evidence of DD-associated treponemes in fresh feces, in slurry, and in hoof lesions by deep sequencing of the V3 and V4 hypervariable regions of the 16S rRNA gene coupled with identification at the operational-taxonomic-unit level. Using treponeme-specific primers in this high-throughput approach, we identified small amounts of DNA (on average 0.6% of the total amount of sequence reads) from DD-associated treponemes in 43 of 64 samples from slurry and cow feces collected from six geographically dispersed dairy herds. Species belonging to the Treponema denticola/Treponema pedis-like and Treponema phagedenis-like phylogenetic clusters were among the most prevalent treponemes in both the dairy herd environment and the DD lesions. By the high-throughput approach presented here, we have demonstrated that cow feces and environmental slurry are possible reservoirs of DD-associated treponemes. This method should enable further clarification of the etiopathogenesis of DD.
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