ArticleApplied and environmental microbiology2014
A tailored galK counterselection system for efficient markerless gene deletion and chromosomal tagging in Magnetospirillum gryphiswaldense.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 45 citations in OpenAlex.
- A cumate-inducible promoter system for tuned gene expression and magnetosome overproduction in magnetotactic bacteria.Applied microbiology and biotechnology · 2026Article
- No evidence for the putative nitric oxide sensor NsrR as a key regulator of magnetosome formation in Magnetospirillum gryphiswaldense.Nucleic acids research · 2026Article
- A Two-Protein Chemoreceptor Complex Regulates Oxygen Thresholds in Bacterial Magneto-Aerotaxis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- MamF-like proteins are distant Tic20 homologs involved in organelle assembly in bacteria.Nature communications · 2024Article
- An Integrated Approach to Elucidate the Interplay between Iron Uptake Dynamics and Magnetosome Formation at the Single-Cell Level inACS applied materials & interfaces · 2024Article
- Reentrant DNA shells tune polyphosphate condensate size.Nature communications · 2024Article
- Reentrant DNA shells tune polyphosphate condensate size.bioRxiv : the preprint server for biology · 2023Article
- Article
- Interacting bactofilins impact cell shape of the MreB-less multicellular Rhodomicrobium vannielii.PLoS genetics · 2023Article
- Silent gene clusters encode magnetic organelle biosynthesis in a non-magnetotactic phototrophic bacterium.The ISME journal · 2023Article
- Effect of oxic and anoxic conditions on intracellular storage of polyhydroxyalkanoate and polyphosphate inFrontiers in microbiology · 2023Article
- The Complex Transcriptional Landscape of Magnetosome Gene Clusters in Magnetospirillum gryphiswaldense.mSystems · 2021Article
- Article
- Identification and elimination of genomic regions irrelevant for magnetosome biosynthesis by large-scale deletion in Magnetospirillum gryphiswaldense.BMC microbiology · 2021Article
- Towards a 'chassis' for bacterial magnetosome biosynthesis: genome streamlining of Magnetospirillum gryphiswaldense by multiple deletions.Microbial cell factories · 2021Article
- Spatiotemporal Organization of Chemotaxis Pathways in Magnetospirillum gryphiswaldense.Applied and environmental microbiology · 2020Article
- Genome-Wide Identification of Essential and Auxiliary Gene Sets for Magnetosome Biosynthesis in Magnetospirillum gryphiswaldense.mSystems · 2020Article
- Acidocalcisomes and Polyphosphate Granules Are Different Subcellular Structures in Agrobacterium tumefaciens.Applied and environmental microbiology · 2020Article
- MamY is a membrane-bound protein that aligns magnetosomes and the motility axis of helical magnetotactic bacteria.Nature microbiology · 2019Article
- Complete genome sequence of Helicobacter pylori B128 7.13 and a single-step method for the generation of unmarked mutations.Helicobacter · 2019Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Magnetotactic bacteria have emerged as excellent model systems to study bacterial cell biology, biomineralization, vesicle formation, and protein targeting because of their ability to synthesize single-domain magnetite crystals within unique organelles (magnetosomes). However, only few species are amenable to genetic manipulation, and the limited methods for site-specific mutagenesis are tedious and time-consuming. Here, we report the adaptation and application of a fast and convenient technique for markerless chromosomal manipulation of Magnetospirillum gryphiswaldense using a single antibiotic resistance cassette and galK-based counterselection for marker recycling. We demonstrate the potential of this technique by genomic excision of the phbCAB operon, encoding enzymes for polyhydroxyalkanoate (PHA) synthesis, followed by chromosomal fusion of magnetosome-associated proteins to fluorescent proteins. Because of the absence of interfering PHA particles, these engineered strains are particularly suitable for microscopic analyses of cell biology and magnetosome biosynthesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.