ArticleGenetics2014
Fitness is strongly influenced by rare mutations of large effect in a microbial mutation accumulation experiment.
Article in Genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 71 citations in OpenAlex.
- Targeted protein evolution in the gut microbiome by diversity-generating retroelements.Science (New York, N.Y.) · 2025Article
- Laboratory evolution of the bacterial genome structure through insertion sequence activation.Nucleic acids research · 2025Article
- Evolutionary dynamics of nascent multicellular lineages.Proceedings. Biological sciences · 2025Article
- Targeted protein evolution in the gut microbiome by diversity-generating retroelements.bioRxiv : the preprint server for biology · 2024Article
- Genus-Wide Transcriptional Landscapes Reveal Correlated Gene Networks Underlying Microevolutionary Divergence in Diatoms.Molecular biology and evolution · 2023Article
- Mutators can drive the evolution of multi-resistance to antibiotics.PLoS genetics · 2023Article
- CDMAP/CDVIS: context-dependent mutation analysis package and visualization software.G3 (Bethesda, Md.) · 2023Article
- Radical shift in the genetic composition of New England chicory populations.The Journal of ecology · 2023Article
- Attempts to limit sporulation in the probiotic strainAccess microbiology · 2023Article
- Are mutations usually deleterious? A perspective on the fitness effects of mutation accumulation.Evolutionary ecology · 2022Article
- Article
- Selection in a growing colony biases results of mutation accumulation experiments.Scientific reports · 2022Article
- The Nutritional Environment Is Sufficient To Select Coexisting Biofilm and Quorum Sensing Mutants of Pseudomonas aeruginosa.Journal of bacteriology · 2022Article
- Precise measurement of the fitness effects of spontaneous mutations by droplet digital PCR in Burkholderia cenocepacia.Genetics · 2021Article
- Self-selection of evolutionary strategies: adaptiveHeliyon · 2021Article
- Evolution of Pseudomonas aeruginosa toward higher fitness under standard laboratory conditions.The ISME journal · 2021Article
- Long-term experimental evolution reveals purifying selection on piRNA-mediated control of transposable element expression.BMC biology · 2020Article
- Population Bottlenecks Strongly Influence the Evolutionary Trajectory to Fluoroquinolone Resistance in Escherichia coli.Molecular biology and evolution · 2020Article
- Article
- Real-time visualization of mutations and their fitness effects in single bacteria.Nature protocols · 2019Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Our understanding of the evolutionary consequences of mutation relies heavily on estimates of the rate and fitness effect of spontaneous mutations generated by mutation accumulation (MA) experiments. We performed a classic MA experiment in which frequent sampling of MA lines was combined with whole genome resequencing to develop a high-resolution picture of the effect of spontaneous mutations in a hypermutator (ΔmutS) strain of the bacterium Pseudomonas aeruginosa. After ∼644 generations of mutation accumulation, MA lines had accumulated an average of 118 mutations, and we found that average fitness across all lines decayed linearly over time. Detailed analyses of the dynamics of fitness change in individual lines revealed that a large fraction of the total decay in fitness (42.3%) was attributable to the fixation of rare, highly deleterious mutations (comprising only 0.5% of fixed mutations). Furthermore, we found that at least 0.64% of mutations were beneficial and probably fixed due to positive selection. The majority of mutations that fixed (82.4%) were base substitutions and we failed to find any signatures of selection on nonsynonymous or intergenic mutations. Short indels made up a much smaller fraction of the mutations that were fixed (17.4%), but we found evidence of strong selection against indels that caused frameshift mutations in coding regions. These results help to quantify the amount of natural selection present in microbial MA experiments and demonstrate that changes in fitness are strongly influenced by rare mutations of large effect.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.