Evidence map›Paper›PMID 24813625›Full record

ArticleNature methods2014

Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography.

Yi-Wei Chang, Songye Chen, Elitza I Tocheva, Anke Treuner-Lange, Stephanie Löbach, Lotte Søgaard-Andersen, Grant J Jensen

Abstract read
In one paragraph

Article in Nature methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 112 papers.

0numbers the graph read from it
0cells of the map it votes in
112citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

112 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
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  5. Article
  6. Review
  7. Review
  8. Single-Molecule 3D Nanoscopy for Application to Cryofixed Samples.The journal of physical chemistry. B · 2025
    Article
  9. Time-deterministic cryo-optical microscopy.Light, science & applications · 2025
    Article
  10. Milestones in the development ofJournal of bacteriology · 2025
    Review
  11. Article
  12. Review
  13. Review
  14. Recent advances in correlative cryo-light and electron microscopy.Current opinion in structural biology · 2024
    Review
  15. Article
  16. bioRxiv : the preprint server for biology · 2024
    Article
  17. Article
  18. Article
  19. Review
  20. Review

52 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yi-Wei Chang1] Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA. [2] Howard Hughes Medical Institute, Pasadena, California, USA.
Songye Chen1] Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA. [2] Howard Hughes Medical Institute, Pasadena, California, USA.
Elitza I TochevaDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.
Anke Treuner-LangeMax Planck Institute for Terrestrial Microbiology, Marburg, Germany.
Stephanie LöbachMax Planck Institute for Terrestrial Microbiology, Marburg, Germany.
Lotte Søgaard-AndersenMax Planck Institute for Terrestrial Microbiology, Marburg, Germany.
Grant J Jensen1] Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA. [2] Howard Hughes Medical Institute, Pasadena, California, USA.

Funding

Structure and function of the prokaryotic cytoskeleton by electron cryotomographyR01GM094800 · NIGMS · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI JENSEN, GRANT J · 2011 to 2015
$1.7M
Howard Hughes Medical InstituteNIGMS NIH HHS R01 GM094800NIGMS NIH HHS R01 GM094800B
6 · The paper itself

Abstract

Cryo-electron tomography (CET) produces three-dimensional images of cells in a near-native state at macromolecular resolution, but identifying structures of interest can be challenging. Here we describe a correlated cryo-PALM (photoactivated localization microscopy)-CET method for localizing objects within cryo-tomograms to beyond the diffraction limit of the light microscope. Using cryo-PALM-CET, we identified multiple and new conformations of the dynamic type VI secretion system in the crowded interior of Myxococcus xanthus.

Indexed as

Bacterial Secretion SystemsCryoelectron MicroscopyElectron Microscope TomographyImaging, Three-DimensionalMyxococcus xanthusBacterial Secretion Systems

Identifiers

PMID24813625
PMCPMC4081473

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.