ArticleJournal of the American Chemical Society2014
Detection and cellular imaging of human cancer enzyme using a turn-on, wavelength-shiftable, self-immolative profluorophore.
Article in Journal of the American Chemical Society, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 183 citations in OpenAlex.
- AND-Logic-Based Dual-Lock Hemicyanine Fluorescence Probe for Accurate Hypoxia Assessment in Tumors.Chemical & biomedical imaging · 2026Article
- Switching on Supramolecular DNA Junction Binding Using a Human Enzyme.Angewandte Chemie (International ed. in English) · 2025Article
- Review
- Enzyme-activatable charge transfer in gold nanoclusters.Chemical science · 2024Article
- Optical substrates for drug-metabolizing enzymes: Recent advances and future perspectives.Acta pharmaceutica Sinica. B · 2022Review
- Activated molecular probes for enzyme recognition and detection.Theranostics · 2022Review
- Enzyme-activated probes in optical imaging: a focus on atherosclerosis.Dalton transactions (Cambridge, England : 2003) · 2021Review
- The diverse functionality of NQO1 and its roles in redox control.Redox biology · 2021Review
- Self-assembled amphiphilic fluorescent probe: detecting pH-fluctuations within cancer cells and tumour tissues.Chemical science · 2020Article
- Light-triggered nitric oxide (NO) release from photoresponsive polymersomes for corneal wound healing.Chemical science · 2020Article
- Engineering an NIR rhodol derivative with spirocyclic ring-opening activation for high-contrast photoacoustic imaging.Chemical science · 2019Article
- Correction: A highly selective fluorescent probe for human NAD(P)H:quinone oxidoreductase 1 (hNQO1) detection and imaging in living tumor cells.RSC advances · 2019Article
- A molecular design strategy toward enzyme-activated probes with near-infrared I and II fluorescence for targeted cancer imaging.Chemical science · 2019Article
- Characterization of a highly specific NQO1-activated near-infrared fluorescent probe and its application for in vivo tumor imaging.Scientific reports · 2019Article
- Dual-biomarker-triggered fluorescence probes for differentiating cancer cells and revealing synergistic antioxidant effects under oxidative stress.Chemical science · 2019Article
- Cancer-Specific Biomarker hNQO1-Activatable Fluorescent Probe for Imaging Cancer Cells In Vitro and In Vivo.Cancers · 2018Article
- Enzyme-Activated Fluorogenic Probes for Live-Cell and in Vivo Imaging.ACS chemical biology · 2018Review
- Detection of DT-diaphorase Enzyme with a ParaCEST MRI Contrast Agent.Chemistry (Weinheim an der Bergstrasse, Germany) · 2017Article
- A Near-Infrared, Wavelength-Shiftable, Turn-on Fluorescent Probe for the Detection and Imaging of Cancer Tumor Cells.ACS chemical biology · 2017Article
- A highly specific ratiometric two-photon fluorescent probe to detect dipeptidyl peptidase IV in plasma and living systems.Biosensors & bioelectronics · 2017Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
A frontier area in the development of activatable (turn-on) fluorescence-based probes is that concerned with rapid and selective stimulus triggering of probe activation so as to allow for biomarker identification and cellular imaging. The work here is concerned with a cloaked fluorophore composed of a reporter whose fluorescence is efficiently quenched by it being bound to an activatable trigger group through a novel self-immolative linker. Highly selective and rapid activation of the trigger group is achieved by chemical and enzymatic means that result in activated trigger group detachment from the self-immolative linker, with the latter subsequently cleaved from the reporter autonomously, thereby unmasking intense, red-shifted fluorescence emission. To achieve this success, we used a trimethyl-locked quinone propionic acid trigger group and an N-methyl-p-aminobenzyl alcohol self-immolative linker attached to the reporter. Delineated here are the synthesis and characterization of this cloaked fluorophore and the evaluation of its triggered turning on in the presence of an up-regulated enzyme in human cancer cells, NAD(P)H: quinone oxidoreductase-1 (NQO1, DT-diaphorase, EC 1.6.99.2).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.