ArticleBioMed research international2014
Detection of Newcastle disease virus minor genetic variants by modified single-stranded conformational polymorphism analysis.
Article in BioMed research international, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 15 citations in OpenAlex.
- Isolation and Molecular Detection of Newcastle Disease Virus From Outbreak Cases in Selected Areas of Northern and Western Oromia, Ethiopia.Veterinary medicine and science · 2026Article
- Newcastle disease burden in Nepal and efficacy of Tablet I2 vaccine in commercial and backyard poultry production.PloS one · 2023Article
- Zoonotic diseases from birds to humans in Vietnam: possible diseases and their associated risk factors.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019Review
- Pathotypic characterization of Newcastle disease virus isolated from vaccinated chicken in West Java, Indonesia.Veterinary world · 2017Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Newcastle disease and Avian Influenza are considered to be the most dangerous fowl diseases which may cause huge economic losses. Newcastle disease is caused by the enveloped, and single-stranded RNA virus (NDV, APMV-1; belonging to Paramyxoviridae family), which can be further divided into sixteen different genotypes grouped into five pathotypes according to their pathogenicity. It has been reported that low pathogenic virus can greatly increase its pathogenicity even during a single passage. Additionally, due to the widespread use of live vaccines, a mixture of two or more different viruses in one sample can be detected. Hence, there is a great need for establishment of fast, inexpensive, sensitive, and relatively simple diagnostic method for multistrain and quasispecies detection of NDV infection. In this paper we describe a diagnostic method based on RT-PCR followed by a modified version of single-stranded conformational polymorphism analysis using short DNA fragments of gene encoding viral F protein. The method allows for rapid diagnosis of genetic variant emerging from previously stable population which may prevent the spread of the pathogenic viral variant.
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