ArticleMolecular and cellular biology1989
Enhancer and silencerlike sites within the transcribed portion of a Ty2 transposable element of Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 36 citations in OpenAlex.
- The Ty1 retrotransposon harbors a DNA region that performs dual functions as both a gene silencing and chromatin insulator.Scientific reports · 2024Article
- The role of wobble uridine modifications in +1 translational frameshifting in eukaryotes.Nucleic acids research · 2015Article
- Glucose signalling pathway controls the programmed ribosomal frameshift efficiency in retroviral-like element Ty3 in Saccharomyces cerevisiae.Yeast (Chichester, England) · 2011Article
- Connection between stop codon reassignment and frequent use of shifty stop frameshifting.RNA (New York, N.Y.) · 2009Article
- Glucose signaling controls the transcription of retrotransposon Ty2-917 in Saccharomyces cerevisiae.Virus genes · 2007Article
- Saturation mutagenesis of a +1 programmed frameshift-inducing mRNA sequence derived from a yeast retrotransposon.RNA (New York, N.Y.) · 2007Article
- An mRNA sequence derived from the yeast EST3 gene stimulates programmed +1 translational frameshifting.RNA (New York, N.Y.) · 2007Article
- An mRNA sequence derived from a programmed frameshifting signal decreases codon discrimination during translation initiation.RNA (New York, N.Y.) · 2006Article
- A downstream regulatory element located within the coding sequence mediates autoregulated expression of the yeast fatty acid synthase gene FAS2 by the FAS1 gene product.Nucleic acids research · 2001Article
- Are all DNA binding and transcription regulation by an activator physiologically relevant?Molecular and cellular biology · 2001Article
- Programmed +1 frameshifting stimulated by complementarity between a downstream mRNA sequence and an error-correcting region of rRNA.RNA (New York, N.Y.) · 2001Article
- Identification and characterisation of two transcriptional repressor elements within the coding sequence of the Saccharomyces cerevisiae HXK2 gene.Nucleic acids research · 1996Article
- Pulling the ribosome out of frame by +1 at a programmed frameshift site by cognate binding of aminoacyl-tRNA.Molecular and cellular biology · 1995Article
- Special peptidyl-tRNA molecules can promote translational frameshifting without slippage.Molecular and cellular biology · 1994Article
- Internally located and oppositely oriented polymerase II promoters direct convergent transcription of a LINE-like retroelement, the Dictyostelium repetitive element, from Dictyostelium discoideum.Molecular and cellular biology · 1994Article
- Involvement of SRE element of Ty1 transposon in TEC1-dependent transcriptional activation.Nucleic acids research · 1994Article
- Yeast intragenic transcriptional control: activation and repression sites within the coding region of the Saccharomyces cerevisiae LPD1 gene.Molecular and cellular biology · 1994Article
- The Drosophila copia retrotransposon contains binding sites for transcriptional regulation by homeoproteins.Nucleic acids research · 1993Article
- Saccharomyces cerevisiae BUF protein binds to sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation.Molecular and cellular biology · 1993Article
- Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The Ty2-917 element is a member of the Ty2 class of retroviruslike transposable elements of Saccharomyces cerevisiae. We showed that regions downstream of the Ty2-917 transcription start site modulate its transcription. One region was located downstream of the transcription initiation site (position 240) and within the first 559 base pairs of the element. This region had a dramatic effect, causing an approximately 1,000-fold increase in steady-state levels of RNA. The region stimulated transcription when placed in either orientation upstream of a heterologous gene, HIS4, lacking its own upstream activation sequence (UAS). We termed this positively acting region an enhancer, by analogy to sites described in higher cells, to distinguish it from yeast UASs which do not function when placed within the transcribed portion of the gene. Though, like some higher eucaryotic enhancers, the Ty2-917 enhancer is located within the transcribed region, it is unlike them in that it occurs within a coding region rather than in an intron. The Ty2-917 enhancer and the Ty2-917 UAS had a synergistic effect on transcription, together stimulating transcription 15-fold over the predicted additive effect. We also identified a site which decreases RNA accumulation, located about 750 base pairs into the element. This site functioned in only one orientation when inserted upstream of the UAS-less heterologous gene. The site was similar to silencers, or negative enhancers, in that it acted to repress transcription from outside the transcribed region, but was distinct in that the function of a canonical silencer was independent of orientation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.