ArticleBiochemistry2014
Insights into the architecture of the eIF2Bα/β/δ regulatory subcomplex.
Article in Biochemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- eIF2B localization and its regulation during the integrated stress response is cell-type specific.iScience · 2024Article
- Molecular glues for protein-protein interactions: Progressing toward a new dream.Cell chemical biology · 2024Review
- Surviving and Adapting to Stress: Translational Control and the Integrated Stress Response.Antioxidants & redox signaling · 2023Review
- eIF2Bδ blocks the integrated stress response and maintains eIF2B activity and cancer metastasis by overexpression in breast cancer stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Regulation and function of elF2B in neurological and metabolic disorders.Bioscience reports · 2022Review
- Sugar phosphate activation of the stress sensor eIF2B.Nature communications · 2021Article
- Characterization and Mapping ofFrontiers in plant science · 2021Article
- Elucidation of the Translation Initiation Factor Interaction Network ofFrontiers in microbiology · 2021Article
- Filament formation by the translation factor eIF2B regulates protein synthesis in starved cells.Biology open · 2020Article
- eIF2B and the Integrated Stress Response: A Structural and Mechanistic View.Biochemistry · 2020Article
- Structure of the nucleotide exchange factor eIF2B reveals mechanism of memory-enhancing molecule.Science (New York, N.Y.) · 2018Article
- eIF2B Mechanisms of Action and Regulation: A Thermodynamic View.Biochemistry · 2018Article
- Article
- Novel mechanisms of eIF2B action and regulation by eIF2α phosphorylation.Nucleic acids research · 2017Article
- Review
- Article
- Expression, purification, and crystallization of Schizosaccharomyces pombe eIF2B.Journal of structural and functional genomics · 2016Article
- Architecture of the eIF2B regulatory subcomplex and its implications for the regulation of guanine nucleotide exchange on eIF2.Nucleic acids research · 2015Article
- Stress responses. Mutations in a translation initiation factor identify the target of a memory-enhancing compound.Science (New York, N.Y.) · 2015Article
- Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Eukaryotic translation initiation factor 2B (eIF2B), the guanine nucleotide exchange factor for the G-protein eIF2, is one of the main targets for the regulation of protein synthesis. The eIF2B activity is inhibited in response to a wide range of stress factors and diseases, including viral infections, hypoxia, nutrient starvation, and heme deficiency, collectively known as the integrated stress response. eIF2B has five subunits (α-ε). The α, β, and δ subunits are homologous to each other and form the eIF2B regulatory subcomplex, which is believed to be a trimer consisting of monomeric α, β, and δ subunits. Here we use a combination of biophysical methods, site-directed mutagenesis, and bioinformatics to show that the human eIF2Bα subunit is in fact a homodimer, at odds with the current trimeric model for the eIF2Bα/β/δ regulatory complex. eIF2Bα dimerizes using the same interface that is found in the homodimeric archaeal eIF2Bα/β/δ homolog aIF2B and related metabolic enzymes. We also present evidence that the eIF2Bβ/δ binding interface is similar to that in the eIF2Bα2 homodimer. Mutations at the predicted eIF2Bβ/δ dimer interface cause genetic neurological disorders in humans. We propose that the eIF2B regulatory subcomplex is an α2β2δ2 hexamer, composed of one α2 homodimer and two βδ heterodimers. Our results offer novel insights into the architecture of eIF2B and its interactions with the G-protein eIF2.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.