ArticleThe Journal of biological chemistry2014
FAD2 and FAD3 desaturases form heterodimers that facilitate metabolic channeling in vivo.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 98 citations in OpenAlex.
- Article
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- Genome-Wide Identification of the Phosphoglycerate Kinases and Functional Analysis ofPlants (Basel, Switzerland) · 2026Article
- Phospholipid Networks as Metabolic Hubs and Signaling Integrators in Plant Development and Stress Adaptation.Plants (Basel, Switzerland) · 2026Review
- Arabidopsis cytochrome b5 proteins support fatty acid ω-3 but not ω-6 desaturation.Plant physiology · 2026Article
- Suppressing wheat sucrose phosphate synthase 1-B protects wheat against stripe rust.Journal of advanced research · 2025Article
- Characterization of fatty acid desaturase gene family inFrontiers in plant science · 2025Article
- 2-Cys peroxiredoxins contribute to thylakoid lipid unsaturation by affecting ω-3 fatty acid desaturase 8.Plant physiology · 2024Article
- Quality assessment of flax advanced breeding lines varying in seed coat color and their potential use in the food and industrial applications.BMC plant biology · 2024Article
- Suppression of ZEAXANTHIN EPOXIDASE 1 restricts stripe rust growth in wheat.Plant communications · 2023Article
- Protein interactomes for plant lipid biosynthesis and their biotechnological applications.Plant biotechnology journal · 2023Review
- Suppressing ASPARTIC PROTEASE 1 prolongs photosynthesis and increases wheat grain weight.Nature plants · 2023Article
- Comparative transcriptomic and evolutionary analysis of FAD-like genes of Brassica species revealed their role in fatty acid biosynthesis and stress tolerance.BMC plant biology · 2023Article
- A near complete genome assembly of chia assists in identification of key fatty acid desaturases in developing seeds.Frontiers in plant science · 2023Article
- Genome-Wide Identification and Expression Analysis of Fatty Acid Desaturase (International journal of molecular sciences · 2022Article
- Membrane fatty acid desaturase: biosynthesis, mechanism, and architecture.Applied microbiology and biotechnology · 2022Review
- Expression of Physaria longchain acyl-CoA synthetases and hydroxy fatty acid accumulation in transgenic Arabidopsis.Journal of plant physiology · 2022Article
- Comparative Analysis and Structural Modeling ofBiology · 2022Article
- Critical metabolic pathways and SAD/FADs, WRI1s, and DGATs cooperate for high-oleic acid oil production in developing oil tea (Horticulture research · 2022Article
- Modification of sesame (Biotechnology reports (Amsterdam, Netherlands) · 2021Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant desaturases comprise two independently evolved classes, a structurally well characterized soluble class responsible for the production of monoenes in the plastids of higher plants and the poorly structurally characterized integral membrane class that has members in the plastid and endoplasmic reticulum that are responsible for producing mono- and polyunsaturated fatty acids. Both require iron and oxygen for activity and are inhibited by azide and cyanide underscoring their common chemical imperatives. We previously showed that the Δ(9) acyl-CoA integral membrane desaturase Ole1p from Saccharomyces cerevisiae exhibits dimeric organization, like the soluble plastidial acyl-ACP desaturases. Here we use two independent bimolecular complementation assays, i.e. yeast two-hybrid analysis and Arabidopsis leaf protoplast split luciferase assay, to demonstrate that members of the plant integral membrane fatty acid desaturase (FAD) family, FAD2, FAD3, FAD6, FAD7, and FAD8, self-associate. Further, the endoplasmic reticulum-localized desaturase FAD2 can associate with FAD3, as can the plastid-localized FAD6 desaturase with either FAD7 or FAD8. These pairings appear to be specific because pairs such as FAD3 and FAD7 (or FAD8) and FAD2 and FAD6 do not interact despite their high amino acid similarity. These results are consistent also with their known endoplasmic reticulum and plastid subcellular localizations. Chemical cross-linking experiments confirm that FAD2 and FAD3 can form dimers like the yeast Ole1p and, when coexpressed, can form FAD2-FAD3 heterodimers. Metabolic flux analysis of yeast coexpressing FAD2 and FAD3 indicates that heterodimers can form a metabolic channel in which 18:1-PC is converted to 18:3-PC without releasing a free 18:2-PC intermediate.
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