ArticleCellular and molecular life sciences : CMLS2014
Fructose transport-deficient Staphylococcus aureus reveals important role of epithelial glucose transporters in limiting sugar-driven bacterial growth in airway surface liquid.
Article in Cellular and molecular life sciences : CMLS, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Effects of Elevated Glucose on Bacterial Respiratory Infections in Cystic Fibrosis and Chronic Airway Diseases.International journal of molecular sciences · 2025Review
- Balancing the dual nature of glycotoxins: interplay of diet, digestion, and gut microbiome.Frontiers in nutrition · 2025Review
- Extragustatory bitter taste receptors in head and neck health and disease.Journal of molecular medicine (Berlin, Germany) · 2024Review
- Diabetes Causes Significant Alterations in Pulmonary Glucose Transporter Expression.Metabolites · 2024Article
- How Does Airway Surface Liquid Composition Vary in Different Pulmonary Diseases, and How Can We Use This Knowledge to Model Microbial Infections?Microorganisms · 2024Review
- Purine and carbohydrate availability drivemBio · 2024Article
- Getting sweeter: new evidence for glucose transporters in specific cell types of the airway?American journal of physiology. Cell physiology · 2023Review
- Mechanistic Insights into the Impact of Air Pollution on Pneumococcal Pathogenesis and Transmission.American journal of respiratory and critical care medicine · 2022Review
- A geroscience perspective on immune resilience and infectious diseases: a potential case for metformin.GeroScience · 2021Review
- Fructose-coated Ångstrom silver prevents sepsis by killing bacteria and attenuating bacterial toxin-induced injuries.Theranostics · 2021Article
- Antimicrobial Peptide Induced-Stress RendersFrontiers in immunology · 2020Article
- Taste Receptors in Upper Airway Innate Immunity.Nutrients · 2019Review
- Taste receptors in the upper airway.World journal of otorhinolaryngology - head and neck surgery · 2018Review
- Differential Effect of LPS on Glucose, Lactate and Inflammatory Markers in the Lungs of Normal and Diabetic Mice.Journal of pulmonary & respiratory medicine · 2017Article
- Dapagliflozin-lowered blood glucose reduces respiratory Pseudomonas aeruginosa infection in diabetic mice.British journal of pharmacology · 2017Article
- Insulin signaling via the PI3-kinase/Akt pathway regulates airway glucose uptake and barrier function in a CFTR-dependent manner.American journal of physiology. Lung cellular and molecular physiology · 2017Article
- Article
- Metformin prevents the effects of Pseudomonas aeruginosa on airway epithelial tight junctions and restricts hyperglycaemia-induced bacterial growth.Journal of cellular and molecular medicine · 2016Article
- Characterization of a novel two-component regulatory system, HptRS, the regulator for the hexose phosphate transport system in Staphylococcus aureus.Infection and immunity · 2015Article
- A novel fluorescent sensor protein for detecting changes in airway surface liquid glucose concentration.The Biochemical journal · 2014Article
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Authors and funding
7 authors at 1 institution in 1 country.
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Abstract
Hyperglycaemia as a result of diabetes mellitus or acute illness is associated with increased susceptibility to respiratory infection with Staphylococcus aureus. Hyperglycaemia increases the concentration of glucose in airway surface liquid (ASL) and promotes the growth of S. aureus in vitro and in vivo. Whether elevation of other sugars in the blood, such as fructose, also results in increased concentrations in ASL is unknown and whether sugars in ASL are directly utilised by S. aureus for growth has not been investigated. We obtained mutant S. aureus JE2 strains with transposon disrupted sugar transport genes. NE768(fruA) exhibited restricted growth in 10 mM fructose. In H441 airway epithelial-bacterial co-culture, elevation of basolateral sugar concentration (5-20 mM) increased the apical growth of JE2. However, sugar-induced growth of NE768(fruA) was significantly less when basolateral fructose rather than glucose was elevated. This is the first experimental evidence to show that S. aureus directly utilises sugars present in the ASL for growth. Interestingly, JE2 growth was promoted less by glucose than fructose. Net transepithelial flux of D-glucose was lower than D-fructose. However, uptake of D-glucose was higher than D-fructose across both apical and basolateral membranes consistent with the presence of GLUT1/10 in the airway epithelium. Therefore, we propose that the preferential uptake of glucose (compared to fructose) limits its accumulation in ASL. Pre-treatment with metformin increased transepithelial resistance and reduced the sugar-dependent growth of S. aureus. Thus, epithelial paracellular permeability and glucose transport mechanisms are vital to maintain low glucose concentration in ASL and limit bacterial nutrient sources as a defence against infection.
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