ArticlePLoS neglected tropical diseases2014
Field-evaluation of a new lateral flow assay for detection of cellular and humoral immunity against Mycobacterium leprae.
Article in PLoS neglected tropical diseases, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 2 of them syntheses that pooled it.
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Who cites it
31 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Leprosy relapse after multidrug therapy: Systematic review and meta-analysis.PLoS neglected tropical diseases · 2025Pooled it
- Pooled it
- Longitudinal assessment of anti-PGL-I serology in contacts of leprosy patients in Bangladesh.PLoS neglected tropical diseases · 2017Trial
- Host biomarker-based quantitative rapid tests for detection and treatment monitoring of tuberculosis and COVID-19.iScience · 2023Article
- Development of lateral flow assays to detect host proteins in cattle for improved diagnosis of bovine tuberculosis.Frontiers in veterinary science · 2023Article
- Article
- Development and validation of a multiplex real-time qPCR assay using GMP-grade reagents for leprosy diagnosis.PLoS neglected tropical diseases · 2022Article
- Quantitative Rapid Test for Detection and Monitoring of Active Pulmonary Tuberculosis in Nonhuman Primates.Biology · 2021Article
- Synthetic Phenolic Glycolipids for Application in Diagnostic Tests for Leprosy.Chembiochem : a European journal of chemical biology · 2021Article
- Recent advances in sensitive surface-enhanced Raman scattering-based lateral flow assay platforms for point-of-care diagnostics of infectious diseases.Sensors and actuators. B, Chemical · 2021Article
- Insights intoProteomes · 2021Review
- Detection and Monitoring ofFrontiers in microbiology · 2021Article
- Novel Diagnostics for Kaposi Sarcoma and Other Skin Diseases in Resource-Limited Settings.Dermatologic clinics · 2021Review
- Transcriptomic Analysis ofFrontiers in cellular and infection microbiology · 2021Article
- Host immune responses induced by specific Mycobacterium leprae antigens in an overnight whole-blood assay correlate with the diagnosis of paucibacillary leprosy patients in China.PLoS neglected tropical diseases · 2019Article
- Inorganic Complexes and Metal-Based Nanomaterials for Infectious Disease Diagnostics.Chemical reviews · 2019Review
- Evaluation of Immunodiagnostic Tests for Leprosy in Brazil, China and Ethiopia.Scientific reports · 2018Article
- [Research advance of lateral flow assay labels].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2018Review
- Specific antigen serologic tests in leprosy: implications for epidemiological surveillance of leprosy cases and household contacts.Memorias do Instituto Oswaldo Cruz · 2017Article
- Field-friendly serological tests for determination of M. leprae-specific antibodies.Scientific reports · 2017Article
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14 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundField-applicable tests detecting asymptomatic Mycobacterium leprae (M. leprae) infection or predicting progression to leprosy, are urgently required. Since the outcome of M. leprae infection is determined by cellular- and humoral immunity, we aim to develop diagnostic tests detecting pro-/anti-inflammatory and regulatory cytokines as well as antibodies against M. leprae. Previously, we developed lateral flow assays (LFA) for detection of cytokines and anti-PGL-I antibodies. Here we evaluate progress of newly developed LFAs for applications in resource-poor settings.
methodsThe combined diagnostic value of IP-10, IL-10 and anti-PGL-I antibodies was tested using M. leprae-stimulated blood of leprosy patients and endemic controls (EC). For reduction of the overall test-to-result time the minimal whole blood assay time required to detect distinctive responses was investigated. To accommodate LFAs for field settings, dry-format LFAs for IP-10 and anti-PGL-I antibodies were developed allowing storage and shipment at ambient temperatures. Additionally, a multiplex LFA-format was applied for simultaneous detection of anti-PGL-I antibodies and IP-10. For improved sensitivity and quantitation upconverting phosphor (UCP) reporter technology was applied in all LFAs.
resultsSingle and multiplex UCP-LFAs correlated well with ELISAs. The performance of dry reagent assays and portable, lightweight UCP-LF strip readers indicated excellent field-robustness. Notably, detection of IP-10 levels in stimulated samples allowed a reduction of the whole blood assay time from 24 h to 6 h. Moreover, IP-10/IL-10 ratios in unstimulated plasma differed significantly between patients and EC, indicating the feasibility to identify M. leprae infection in endemic areas.
conclusionsDry-format UCP-LFAs are low-tech, robust assays allowing detection of relevant cytokines and antibodies in response to M. leprae in the field. The high levels of IP-10 and the required shorter whole blood assay time, render this cytokine useful to discriminate between leprosy patients and EC.
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