ArticleFEMS microbiology letters2014
An autoinhibitory conformation of the Bacillus subtilis spore coat protein SpoIVA prevents its premature ATP-independent aggregation.
Article in FEMS microbiology letters, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 26 citations in OpenAlex.
- 16S ribosomal RNA modification drives transcript-specific translation efficiency.Cell reports · 2026Article
- A lipid cue drives the subcellular localization of a self-inserting bacterial transmembrane protein.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- A lipid cue drives the subcellular localization of a self-inserting bacterial transmembrane protein.bioRxiv : the preprint server for biology · 2026Article
- 16S ribosomal RNA modification drives transcript-specific translation efficiency.bioRxiv : the preprint server for biology · 2026Article
- Article
- To Sporulate or Not to Sporulate: Developmental Checkpoints MonitoringAnnual review of microbiology · 2025Review
- Bacterial spore surface nanoenvironment requires a AAA+ ATPase to promote MurG function.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Altruistic feeding and cell-cell signaling during bacterial differentiation actively enhance phenotypic heterogeneity.Science advances · 2024Article
- Altruistic feeding and cell-cell signaling during bacterial differentiation actively enhance phenotypic heterogeneity.bioRxiv : the preprint server for biology · 2024Article
- SpoIVA is an essential morphogenetic protein for the formation of heat- and lysozyme-resistant spores inFrontiers in microbiology · 2024Article
- Bacterial cell surface nanoenvironment requires a specialized chaperone to activate a peptidoglycan biosynthetic enzyme.bioRxiv : the preprint server for biology · 2023Article
- Cell-specific cargo delivery using synthetic bacterial spores.Cell reports · 2023Article
- Article
- Role of SpoIVA ATPase Motifs during Clostridioides difficile Sporulation.Journal of bacteriology · 2020Article
- A 2-dimensional ratchet model describes assembly initiation of a specialized bacterial cell surface.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Vaccine display on artificial bacterial spores enhances protective efficacy against Staphylococcus aureus infection.FEMS microbiology letters · 2018Article
- Cell Death Pathway That Monitors Spore Morphogenesis.Trends in microbiology · 2017Review
- A Quality-Control Mechanism Removes Unfit Cells from a Population of Sporulating Bacteria.Developmental cell · 2015Article
- Structural basis for the geometry-driven localization of a small protein.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- A versatile nano display platform from bacterial spore coat proteins.Nature communications · 2015Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Spores of Bacillus subtilis are dormant cell types that are formed when the bacterium encounters starvation conditions. Spores are encased in a shell, termed the coat, which is composed of approximately seventy different proteins and protects the spore's genetic material from environmental insults. The structural component of the basement layer of the coat is an exceptional cytoskeletal protein, termed SpoIVA, which binds and hydrolyzes ATP. ATP hydrolysis is utilized to drive a conformational change in SpoIVA that leads to its irreversible self-assembly into a static polymer in vitro. Here, we characterize the middle domain of SpoIVA, the predicted secondary structure of which resembles the chemotaxis protein CheX but, unlike CheX, does not harbor residues required for phosphatase activity. Disruptions in this domain did not abolish ATP hydrolysis, but resulted in mislocalization of the protein and reduction in sporulation efficiency in vivo. In vitro, disruptions in this domain prevented the ATP hydrolysis-driven conformational change in SpoIVA required for polymerization and led to the aggregation of SpoIVA into particles that did not form filaments. We propose a model in which SpoIVA initially assumes a conformation in which it inhibits its own aggregation into particles, and that ATP hydrolysis remodels the protein so that it assumes a polymerization-competent conformation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.