ArticleScientific reports2014
Establishment and application of a loop-mediated isothermal amplification (LAMP) system for detection of cry1Ac transgenic sugarcane.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 110 citations in OpenAlex.
- Rapid Point-of-Care Detection ofAnimals : an open access journal from MDPI · 2026Article
- A rapid analytical method using loop-mediated isothermal amplification (LAMP): visual detection of cry1Ab gene in genetically modified maize seeds and food products.Biotechnology letters · 2026Article
- Genome-wide analysis of the sugarcane SUT gene family reveals ShSUT4 as a key regulator of abiotic stress responses.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Comparative evaluation of 14Frontiers in microbiology · 2026Article
- Development and validation of LAMP and real-time LAMP assays for rapid detection of coccinia mosaic Virudhunagar virus in bitter gourd (3 Biotech · 2025Article
- Loop-Mediated Isothermal Amplification (LAMP) Genotyping for Presence-Absence Determination of Gene of Interest in Test Samples.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Comparative assessment of two in-house-built isothermal assays for visual detection of African swine fever virus.Journal of biosciences · 2024Article
- Current and Future Advances in the Detection and Surveillance of Biosecurity-Relevant Equine Bacterial Diseases Using Loop-Mediated Isothermal Amplification (LAMP).Animals : an open access journal from MDPI · 2023Review
- Rapid Detection of Hepatitis A Virus in Foods Using a Bioluminescent Assay in Real-Time (BART) and Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Technology.Food and environmental virology · 2023Article
- Rapid identification of Staphylococcus aureus based on a fluorescence imaging/detection platform that combines loop mediated isothermal amplification assay and the smartphone-based system.Scientific reports · 2022Article
- Simple, sensitive, and cost-effective detection of wAlbB Wolbachia in Aedes mosquitoes, using loop mediated isothermal amplification combined with the electrochemical biosensing method.PLoS neglected tropical diseases · 2022Article
- Loop-Mediated Isothermal Amplification for Detection of Plant Pathogens in Wheat (Frontiers in plant science · 2022Review
- A paper-based colorimetric molecular test for SARS-CoV-2 in saliva.Biosensors & bioelectronics: X · 2021Article
- "Shining a LAMP" (Loop-Mediated Isothermal Amplification) on the Molecular Detection of PhytopathogensPathogens (Basel, Switzerland) · 2021Article
- Resistance to the SDHI Fungicides Boscalid and Fluopyram inJournal of fungi (Basel, Switzerland) · 2021Article
- Development of loop-mediated isothermal amplification (LAMP) assay using SYBR safe and gold-nanoparticle probe for detection of Leishmania in HIV patients.Scientific reports · 2021Article
- Development and validation of a one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) for rapid detection of ZIKV in patient samples from Brazil.Scientific reports · 2021Article
- Rapid and Specific Detection of the Poplar Black Spot Disease Caused byPlants (Basel, Switzerland) · 2021Article
- DjinniChip: evaluation of a novel molecular rapid diagnostic device for the detection of Chlamydia trachomatis in trachoma-endemic areas.Parasites & vectors · 2020Article
- A flexible format LAMP assay for rapid detection of Ebola virus.PLoS neglected tropical diseases · 2020Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To meet the demand for detection of foreign genes in genetically modified (GM) sugarcane necessary for regulation of gene technology, an efficient method with high specificity and rapidity was developed for the cry1Ac gene, based on loop-mediated isothermal amplification (LAMP). A set of four primers was designed using the sequence of cry1Ac along with optimized reaction conditions: 5.25 mM of Mg(2+), 4:1 ratio of inner primer to outer primer, 2.0 U of Bst DNA polymerase in a reaction volume of 25.0 μL. Three post-LAMP detection methods (precipitation, calcein (0.60 mM) with Mn(2+) (0.05 mM) complex and SYBR Green I visualization), were shown to be effective. The sensitivity of the LAMP method was tenfold higher than that of conventional PCR when using templates of the recombinant cry1Ac plasmid or genomic DNA from cry1Ac transgenic sugarcane plants. More importantly, this system allowed detection of the foreign gene on-site when screening GM sugarcane without complex and expensive instruments, using the naked eye. This method can not only provide technological support for detection of cry1Ac, but can also further facilitate the use of this detection technique for other transgenes in GM sugarcane.
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