ArticlePLoS pathogens2014
SslE elicits functional antibodies that impair in vitro mucinase activity and in vivo colonization by both intestinal and extraintestinal Escherichia coli strains.
Article in PLoS pathogens, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 43 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
43 citing papers in PubMed, 66 citations in OpenAlex.
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- Roles of Glycoconjugates and Mucinases from Carcinogenic Liver Fluke, Opisthorchis viverrini, in Host: Parasite Interactions.Advances in experimental medicine and biology · 2026Review
- Mucinase fromFrontiers in microbiology · 2026Article
- Systemic IgG responses to glycosylated mucinase YghJ after experimental enterotoxigenic Escherichia coli infection.Gut pathogens · 2025Article
- Characterization of bacteria colonizing the mucosal layer of the gastrointestinal tract of Atlantic salmon farmed in a warm water region.Frontiers in microbiology · 2025Article
- Less is more: the lack of autoinducer-2-dependent quorum sensing promotes competitive fitness ofFrontiers in cellular and infection microbiology · 2025Article
- Proportions of IgA antibodies targeting glycosylated epitopes of secreted Escherichia coli mucinase YghJ in initial plasmablast response differ from salivary and intestinally secreted IgA.Medical microbiology and immunology · 2024Article
- A Comprehensive Review Exploring the Protective Role of Specific Commensal Gut Bacteria againstPathogens (Basel, Switzerland) · 2024Review
- Identification of a genomic cluster related to hypersecretion of intestinal mucus and mucinolytic activity of atypical enteropathogenicFrontiers in cellular and infection microbiology · 2024Article
- Isolation, identification, molecular typing, and drug resistance of Escherichia coli from infected cattle and sheep in Xinjiang, China.Veterinary medicine and science · 2023Article
- O-Mucin-degrading carbohydrate-active enzymes and their possible implication in inflammatory bowel diseases.Essays in biochemistry · 2023Review
- Glycosylation of bacterial antigens changes epitope patterns.Frontiers in immunology · 2023Article
- Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation.NPJ biofilms and microbiomes · 2022Article
- Novel Simple Conjugation Chemistries for Decoration of GMMA with Heterologous Antigens.International journal of molecular sciences · 2021Article
- Foxo1 controls gut homeostasis and commensalism by regulating mucus secretion.The Journal of experimental medicine · 2021Article
- Vaccines for Protecting Infants from Bacterial Causes of Diarrheal Disease.Microorganisms · 2021Review
- Confronting challenges to enterotoxigenicFrontiers in tropical diseases · 2021Article
- Linking inherent O-Linked Protein Glycosylation of YghJ to Increased Antigen Potential.Frontiers in cellular and infection microbiology · 2021Article
- The transcription regulator and c-di-GMP phosphodiesterase PdeL represses motility inJournal of bacteriology · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
23 authors at 6 institutions in 5 countries.
Funding
Abstract
SslE, the Secreted and surface-associated lipoprotein from Escherichia coli, has recently been associated to the M60-like extracellular zinc-metalloprotease sub-family which is implicated in glycan recognition and processing. SslE can be divided into two main variants and we recently proposed it as a potential vaccine candidate. By applying a number of in vitro bioassays and comparing wild type, knockout mutant and complemented strains, we have now demonstrated that SslE specifically contributes to degradation of mucin substrates, typically present in the intestine and bladder. Mutation of the zinc metallopeptidase motif of SslE dramatically impaired E. coli mucinase activity, confirming the specificity of the phenotype observed. Moreover, antibodies raised against variant I SslE, cloned from strain IHE3034 (SslEIHE3034), are able to inhibit translocation of E. coli strains expressing different variants through a mucin-based matrix, suggesting that SslE induces cross-reactive functional antibodies that affect the metallopeptidase activity. To test this hypothesis, we used well-established animal models and demonstrated that immunization with SslEIHE3034 significantly reduced gut, kidney and spleen colonization by strains producing variant II SslE and belonging to different pathotypes. Taken together, these data strongly support the importance of SslE in E. coli colonization of mucosal surfaces and reinforce the use of this antigen as a component of a broadly protective vaccine against pathogenic E. coli species.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.