ArticleTechnology2013
Measuring neutrophil speed and directionality during chemotaxis, directly from a droplet of whole blood.
Article in Technology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 55 citations in OpenAlex.
- Communication through autoattractants can enhance and limit collective migration of immune cells.PLoS computational biology · 2026Article
- Article
- [Inertial label-free sorting and chemotaxis of polymorphonuclear neutrophil in sepsis patients based on microfluidic technology].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2023Article
- Passive redirection filters minimize red blood cell contamination during neutrophil chemotaxis assays using whole blood.Lab on a chip · 2023Article
- Simplified Cell Magnetic Isolation Assisted SCMicromachines · 2022Article
- Integrative experimental/computational approach establishes active cellular protrusion as the primary driving force of phagocytic spreading by immune cells.PLoS computational biology · 2022Article
- Multifactorial assessment of neutrophil chemotaxis efficiency from a drop of blood.Journal of leukocyte biology · 2022Article
- Method for simultaneous tracking of thousands of unlabeled cells within a transparent 3D matrix.PloS one · 2022Article
- Article
- Hybrid models of chemotaxis with application to leukocyte migration.Journal of mathematical biology · 2021Article
- Neutrophils: Need for Standardized Nomenclature.Frontiers in immunology · 2021Review
- Electrotaxis-on-Chip to Quantify Neutrophil Migration Towards Electrochemical Gradients.Frontiers in immunology · 2021Article
- Membrane Tension Can Enhance Adaptation to Maintain Polarity of Migrating Cells.Biophysical journal · 2020Article
- L-Arginine Modulates Neonatal Leukocyte Recruitment in a Gestational Age-Dependent Manner.Journal of clinical medicine · 2020Article
- Microfluidic devices for neutrophil chemotaxis studies.Journal of translational medicine · 2020Review
- Combinatorial nanodot stripe assay to systematically study cell haptotaxis.Microsystems & nanoengineering · 2020Article
- Inflammation-on-a-Chip: Probing the Immune System Ex Vivo.Trends in biotechnology · 2018Review
- Presurgical localization of infected avascular bone segments in chronic complicated posttraumatic osteomyelitis in the lower extremity using dual-tracer PET/CT.EJNMMI research · 2018Article
- Diagnosis of sepsis from a drop of blood by measurement of spontaneous neutrophil motility in a microfluidic assay.Nature biomedical engineering · 2018Article
- A computational model for how cells choose temporal or spatial sensing during chemotaxis.PLoS computational biology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Neutrophil chemotaxis is critical for defense against infections and its alterations could lead to chronic inflammation and tissue injury. The central role that transient alterations of neutrophil chemotaxis could have on patient outcomes calls for its quantification in the clinic. However, current methods for measuring neutrophil chemotaxis require large volumes of blood and are time consuming. To address the need for rapid and robust assays, we designed a microfluidic device that measures neutrophil chemotaxis directly from a single droplet of blood. We validated the assay by comparing neutrophil chemotaxis from finger prick, venous blood and purified neutrophil samples. We found consistent average velocity of (19 ± 6 μm/min) and directionality (91.1%) between the three sources. We quantified the variability in neutrophil chemotaxis between healthy donors and found no significant changes over time. We also validated the device in the clinic and documented temporary chemotaxis deficiencies after burn injuries.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.