ArticleJournal of clinical microbiology2014
Evaluation of three rapid diagnostic methods for direct identification of microorganisms in positive blood cultures.
Article in Journal of clinical microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 1 synthesis or guideline pooled it, 108 citations in OpenAlex.
- Clinical Utility of Molecular Tests for Guiding Therapeutic Decisions in Bloodstream Staphylococcal Infections: A Meta-Analysis.Frontiers in pediatrics · 2021Pooled it
- Evaluating the Cost Effectiveness of Rapid Diagnostic Testing for the Identification of Pathogens and Resistance Genes in Bloodstream Infections.PharmacoEconomics · 2026Article
- Clinical and Economic Value of Rapid Microbiological Diagnostics in Bloodstream Infections: A State-of-the-Art Evidence Review with Emphasis on PCR and MALDI-TOF.Microorganisms · 2026Review
- Accuracy of two Sepsityper MALDI-TOF MS methods for bacterial identification in bloodstream infections in dogs, foals, and calves using Bayesian latent class model.The veterinary quarterly · 2025Article
- A comparative study using Xpert MTB/RIF and culture methods evaluates MassARRAY technology for rapid detection ofFrontiers in cellular and infection microbiology · 2025Article
- Comparison of 3 diagnostic platforms for identification of bacteria and yeast from positive blood culture bottles.Diagnostic microbiology and infectious disease · 2023Article
- CPA-Cas12a-based lateral flow strip for portable assay of Methicillin-resistant Staphylococcus aureus in clinical sample.Journal of nanobiotechnology · 2023Article
- A Closer Look at the Laboratory Impact of Utilizing ePlex Blood Culture Identification Panels: a Workflow Analysis Using Rapid Molecular Detection for Positive Blood Cultures.Microbiology spectrum · 2022Article
- Metabolic preference assay for rapid diagnosis of bloodstream infections.Nature communications · 2022Article
- Identification and Antibiotic Susceptibility Patterns of Clinical Blood Culture Isolates Not Identified by a Rapid Microarray Diagnostic System.Microbiology spectrum · 2021Observational
- Chondroprotective effects of purple corn anthocyanins on advanced glycation end products induction through suppression of NF-κB and MAPK signaling.Scientific reports · 2021Article
- Evaluation of Rapid Sepsityper® protocol and specific MBT-Sepsityper module (Bruker Daltonics) for the rapid diagnosis of bacteremia and fungemia by MALDI-TOF-MS.Annals of clinical microbiology and antimicrobials · 2020Article
- Identification and antimicrobial susceptibility testing of Gram-positive and Gram-negative bacteria from positive blood cultures using the Accelerate Pheno™ system.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Article
- A new culture-based method for rapid identification of microorganisms in polymicrobial blood cultures by MALDI-TOF MS.BMC microbiology · 2019Article
- Article
- Emerging Analytical Techniques for Rapid Pathogen Identification and Susceptibility Testing.Annual review of analytical chemistry (Palo Alto, Calif.) · 2019Review
- Use of the MALDI BioTyper system and rapid BACpro with MALDI-TOF MS for rapid identification of microorganisms causing bacterial urinary tract infection in feline urine samples.The Journal of veterinary medical science · 2018Article
- Simple Sample Preparation Method for Direct Microbial Identification and Susceptibility Testing From Positive Blood Cultures.Frontiers in microbiology · 2018Article
- Parallel Evaluation of the MALDI Sepsityper and Verigene BC-GN Assays for Rapid Identification of Gram-Negative Bacilli from Positive Blood Cultures.Journal of clinical microbiology · 2017Article
- Multicenter Evaluation of the Portrait Staph ID/R Blood Culture Panel for Rapid Identification of Staphylococci and Detection of theJournal of clinical microbiology · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The identification of organisms from positive blood cultures generally takes several days. However, recently developed rapid diagnostic methods offer the potential for organism identification within only a few hours of blood culture positivity. In this study, we evaluated the performance of three commercial methods to rapidly identify organisms directly from positive blood cultures: QuickFISH (AdvanDx, Wolburn, MA), Verigene Gram-Positive Blood Culture (BC-GP; Nanosphere, Northbrook, IL), and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) with Sepsityper processing (Bruker Daltonics, Billerica, MA). A total of 159 blood cultures (VersaTREK Trek Diagnostic Systems, Cleveland, OH) positive for Gram-positive and Gram-negative bacteria as well as yeast were analyzed with QuickFISH and MALDI-TOF MS. In all, 102 blood cultures were analyzed using the BC-GP assay. For monomicrobial cultures, we observed 98.0% concordance with routine methods for both QuickFISH (143/146) and the BC-GP assay (93/95). MALDI-TOF MS demonstrated 80.1% (117/146) and 87.7% (128/146) concordance with routine methods to the genus and species levels, respectively. None of the methods tested were capable of consistently identifying polymicrobial cultures in their entirety or reliably differentiating Streptococcus pneumoniae from viridans streptococci. Nevertheless, the methods evaluated in this study are convenient and accurate for the most commonly encountered pathogens and have the potential to dramatically reduce turnaround time for the provision of results to the treating physician.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.