ArticleMolecular biology of the cell2014
Systematic spatial mapping of proteins at exocytic and endocytic structures.
Article in Molecular biology of the cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Spatial and signaling overlap of growth factor receptor systems at clathrin-coated sites.Molecular biology of the cell · 2024Article
- Crosstalk of growth factor receptors at plasma membrane clathrin-coated sites.bioRxiv : the preprint server for biology · 2024Article
- Dual clathrin and integrin signaling systems regulate growth factor receptor activation.Nature communications · 2022Article
- Schizophrenia-Linked Protein tSNARE1 Regulates Endosomal Trafficking in Cortical Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- The nanoscale molecular morphology of docked exocytic dense-core vesicles in neuroendocrine cells.Nature communications · 2021Article
- The structure and spontaneous curvature of clathrin lattices at the plasma membrane.Developmental cell · 2021Article
- Structurally distinct endocytic pathways for B cell receptors in B lymphocytes.Molecular biology of the cell · 2020Article
- Imaging the rapid yet transient accumulation of regulatory lipids, lipid kinases, and protein kinases during membrane fusion, at sites of exocytosis of MMP-9 in MCF-7 cells.Lipids in health and disease · 2020Article
- Determining the dynamics of cancer cell secretion.The Journal of general physiology · 2019Article
- Spatiotemporal organization and protein dynamics involved in regulated exocytosis of MMP-9 in breast cancer cells.The Journal of general physiology · 2019Article
- Simple differences in the protein-membrane attachment mechanism have functional consequences for surface mechanics.The Journal of chemical physics · 2019Article
- A primer on resolving the nanoscale structure of the plasma membrane with light and electron microscopy.The Journal of general physiology · 2019Review
- Article
- Mapping molecular assemblies with fluorescence microscopy and object-based spatial statistics.Nature communications · 2018Article
- Membrane bending occurs at all stages of clathrin-coat assembly and defines endocytic dynamics.Nature communications · 2018Article
- Article
- Clathrin polymerization exhibits high mechano-geometric sensitivity.Soft matter · 2017Article
- Imaging the recruitment and loss of proteins and lipids at single sites of calcium-triggered exocytosis.Molecular biology of the cell · 2016Article
- Transient Fcho1/2⋅Eps15/R⋅AP-2 Nanoclusters Prime the AP-2 Clathrin Adaptor for Cargo Binding.Developmental cell · 2016Article
- Resident CAPS on dense-core vesicles docks and primes vesicles for fusion.Molecular biology of the cell · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Vesicular secretion (exocytosis) involves the release and then compensatory recycling of vesicle components through endocytosis. This fundamental cellular process is controlled by the coordinated assembly and interactions of dozens of proteins at the plasma membrane. Understanding the molecular composition of individual exocytic and endocytic structures and their organization across the plasma membrane is critical to understanding the behavior and regulation of these two cellular processes. Here we develop a high-resolution and high-throughput fluorescence imaging-based approach for the unbiased mapping of 78 proteins at single exocytic vesicles and endocytic structures in neuroendocrine PC12 cells. This analysis uses two-color single-frame images to provide a systems-level map of the steady-state distributions of proteins at individual exocytic and endocytic structures in the cell. Along with this quantitative map, we find that both calcium-regulated exocytic vesicles (dense core vesicles) and endocytic structures (clathrin-coated structures) and the proteins associated with these structures exhibit a random spatial distribution in unstimulated neuroendocrine PC12 cells. This approach is broadly applicable for quantitatively mapping the molecular composition and spatial organization of discrete cellular processes with central molecular hubs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.