ArticleMolecular biology of the cell2014
Kinetochore-microtubule stability governs the metaphase requirement for Eg5.
Article in Molecular biology of the cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 48 citations in OpenAlex.
- Kinesin Spindle Protein (KIF11) in Mitosis and Cancer.International journal of molecular sciences · 2025Review
- Synergy between inhibitors of two mitotic spindle assembly motors undermines an adaptive response.Molecular biology of the cell · 2022Article
- EML2-S constitutes a new class of proteins that recognizes and regulates the dynamics of tyrosinated microtubules.Current biology : CB · 2022Article
- Kinesin-5 Eg5 mediates centrosome separation to control spindle assembly in spermatocytes.Chromosoma · 2022Article
- More than two populations of microtubules comprise the dynamic mitotic spindle.Journal of cell science · 2022Article
- Kinesin-14 HSET may not oppose kinesin-5 Eg5 activity in RPE-1 cells.microPublication biology · 2022Article
- K-fiber bundles in the mitotic spindle are mechanically reinforced by Kif15.Molecular biology of the cell · 2021Article
- Retinal Features of Family Members With Familial Exudative Vitreoretinopathy Caused By Mutations in KIF11 Gene.Translational vision science & technology · 2021Article
- Microtubule-sliding modules based on kinesins EG5 and PRC1-dependent KIF4A drive human spindle elongation.Developmental cell · 2021Article
- Parthenolide Destabilizes Microtubules by Covalently Modifying Tubulin.Current biology : CB · 2021Article
- The Microtubule Cytoskeleton during the EarlyCells · 2020Article
- HSP70 regulates Eg5 distribution within the mitotic spindle and modulates the cytotoxicity of Eg5 inhibitors.Cell death & disease · 2020Article
- A mouse model for kinesin family member 11 (Kif11)-associated familial exudative vitreoretinopathy.Human molecular genetics · 2020Article
- Review
- NuMA assemblies organize microtubule asters to establish spindle bipolarity in acentrosomal human cells.The EMBO journal · 2020Article
- Kinesin-5 Eg5 is essential for spindle assembly and chromosome alignment of mouse spermatocytes.Cell division · 2020Article
- A comparative analysis of methods to measure kinetochore-microtubule attachment stability.Methods in cell biology · 2020Article
- The evolution of spindles and their mechanical implications for cancer metastasis.Cell cycle (Georgetown, Tex.) · 2019Article
- Helical Twist and Rotational Forces in the Mitotic Spindle.Biomolecules · 2019Review
- The Spindle: Integrating Architecture and Mechanics across Scales.Trends in cell biology · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The mitotic spindle is a bipolar, microtubule (MT)-based cellular machine that segregates the duplicated genome into two daughter cells. The kinesin-5 Eg5 establishes the bipolar geometry of the mitotic spindle, but previous work in mammalian cells suggested that this motor is unimportant for the maintenance of spindle bipolarity. Although it is known that Kif15, a second mitotic kinesin, enforces spindle bipolarity in the absence of Eg5, how Kif15 functions in this capacity and/or whether other biochemical or physical properties of the spindle promote its bipolarity have been poorly studied. Here we report that not all human cell lines can efficiently maintain bipolarity without Eg5, despite their expressing Kif15. We show that the stability of chromosome-attached kinetochore-MTs (K-MTs) is important for bipolar spindle maintenance without Eg5. Cells that efficiently maintain bipolar spindles without Eg5 have more stable K-MTs than those that collapse without Eg5. Consistent with this observation, artificial destabilization of K-MTs promotes spindle collapse without Eg5, whereas stabilizing K-MTs improves bipolar spindle maintenance without Eg5. Our findings suggest that either rapid K-MT turnover pulls poles inward or slow K-MT turnover allows for greater resistance to inward-directed forces.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.