ArticleBiophysical journal2014
Single-molecule spectroscopy unmasks the lowest exciton state of the B850 assembly in LH2 from Rps. acidophila.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed.
- Quantum chemical elucidation of a sevenfold symmetric bacterial antenna complex.Photosynthesis research · 2023Article
- Absorption and Circular Dichroism Spectra of Molecular Aggregates With the Full Cumulant Expansion.The journal of physical chemistry. B · 2020Article
- Hole-Burning Spectroscopy on Excitonically Coupled Pigments in Proteins: Theory Meets Experiment.Journal of the American Chemical Society · 2016Article
- Broadband single-molecule excitation spectroscopy.Nature communications · 2016Article
- Fluorescence polarization measures energy funneling in single light-harvesting antennas--LH2 vs conjugated polymers.Scientific reports · 2015Article
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6 authors.
Funding
Abstract
We have recorded fluorescence-excitation and emission spectra from single LH2 complexes from Rhodopseudomonas (Rps.) acidophila. Both types of spectra show strong temporal spectral fluctuations that can be visualized as spectral diffusion plots. Comparison of the excitation and emission spectra reveals that for most of the complexes the lowest exciton transition is not observable in the excitation spectra due to the cutoff of the detection filter characteristics. However, from the spectral diffusion plots we have the full spectral and temporal information at hand and can select those complexes for which the excitation spectra are complete. Correlating the red most spectral feature of the excitation spectrum with the blue most spectral feature of the emission spectrum allows an unambiguous assignment of the lowest exciton state. Hence, application of fluorescence-excitation and emission spectroscopy on the same individual LH2 complex allows us to decipher spectral subtleties that are usually hidden in traditional ensemble spectroscopy.
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