ArticleJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research2014
Botulinum toxin induces muscle paralysis and inhibits bone regeneration in zebrafish.
Article in Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 42 citations in OpenAlex.
- Botulinum toxin type A injection into the masticatory muscles and its effects on mandibular bone resorption and density. A systematic review.Clinical oral investigations · 2024Pooled it
- Zebrafish Models for Skeletal and Extraskeletal Osteogenesis Imperfecta Features: Unveiling Pathophysiology and Paving the Way for Drug Discovery.Calcified tissue international · 2024Review
- Disruption of the creb3l1 gene causes defects in caudal fin regeneration and patterning in zebrafish Danio rerio.Developmental dynamics : an official publication of the American Association of Anatomists · 2024Article
- Fin ray branching is defined by TRAPProceedings of the National Academy of Sciences of the United States of America · 2022Article
- wnt16 regulates spine and muscle morphogenesis through parallel signals from notochord and dermomyotome.PLoS genetics · 2022Article
- Digit specific denervation does not inhibit mouse digit tip regeneration.Developmental biology · 2022Article
- Osteoblasts pattern endothelium and somatosensory axons during zebrafish caudal fin organogenesis.Development (Cambridge, England) · 2022Article
- Mouse Digit Tip Regeneration Is Mechanical Load Dependent.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2022Article
- Bone Phenotyping Approaches in Human, Mice and Zebrafish - Expert Overview of the EU Cost Action GEMSTONE ("GEnomics of MusculoSkeletal traits TranslatiOnal NEtwork").Frontiers in endocrinology · 2021Review
- Fish Models of Induced Osteoporosis.Frontiers in cell and developmental biology · 2021Review
- Zebrafish: An Emerging Model for Orthopedic Research.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2020Review
- Ageing impacts phenotypic flexibility in an air-acclimated amphibious fish.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2019Article
- Skeletal Stem Cell-Schwann Cell Circuitry in Mandibular Repair.Cell reports · 2019Article
- Using zebrafish to study skeletal genomics.Bone · 2019Review
- Zebrafish as an Emerging Model for Osteoporosis: A Primary Testing Platform for Screening New Osteo-Active Compounds.Frontiers in endocrinology · 2019Review
- Tendon healing in the context of complex fractures.Clinical reviews in bone and mineral metabolism · 2018Article
- ScreenCube: A 3D Printed System for Rapid and Cost-Effective Chemical Screening in Adult Zebrafish.Zebrafish · 2018Article
- The brain is required for normal muscle and nerve patterning during early Xenopus development.Nature communications · 2017Article
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Authors and funding
15 authors at 2 institutions in 1 country.
Funding
Abstract
Intramuscular administration of Botulinum toxin (BTx) has been associated with impaired osteogenesis in diverse conditions of bone formation (eg, development, growth, and healing), yet the mechanisms of neuromuscular-bone crosstalk underlying these deficits have yet to be identified. Motivated by the emerging utility of zebrafish (Danio rerio) as a rapid, genetically tractable, and optically transparent model for human pathologies (as well as the potential to interrogate neuromuscular-mediated bone disorders in a simple model that bridges in vitro and more complex in vivo model systems), in this study, we developed a model of BTx-induced muscle paralysis in adult zebrafish, and we examined its effects on intramembranous ossification during tail fin regeneration. BTx administration induced rapid muscle paralysis in adult zebrafish in a manner that was dose-dependent, transient, and focal, mirroring the paralytic phenotype observed in animal and human studies. During fin regeneration, BTx impaired continued bone ray outgrowth, morphology, and patterning, indicating defects in early osteogenesis. Further, BTx significantly decreased mineralizing activity and crystalline mineral accumulation, suggesting delayed late-stage osteoblast differentiation and/or altered secondary bone apposition. Bone ray transection proximal to the amputation site focally inhibited bone outgrowth in the affected ray, implicating intra- and/or inter-ray nerves in this process. Taken together, these studies demonstrate the potential to interrogate pathological features of BTx-induced osteoanabolic dysfunction in the regenerating zebrafish fin, define the technological toolbox for detecting bone growth and mineralization deficits in this process, and suggest that pathways mediating neuromuscular regulation of osteogenesis may be conserved beyond established mammalian models of bone anabolic disorders.
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