Evidence map›Paper›PMID 24806462›Full record

ArticlePloS one2014

A simple and versatile 2-dimensional platform to study plant germination and growth under controlled humidity.

Tom Sizmur, Kara R Lind, Saida Benomar, Hannah VanEvery, Ludovico Cademartiri

Abstract read
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Evidence for root adaptation to a spatially discontinuous water availability in the absence of external water potential gradients.Proceedings of the National Academy of Sciences of the United States of America · 2021
    Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Tom SizmurDepartment of Materials Science & Engineering, Iowa State University, Ames, Iowa, United States of America; Ames Laboratory, US Department of Energy, Iowa State University, Ames, Iowa, United States of America.
Kara R LindDepartment of Materials Science & Engineering, Iowa State University, Ames, Iowa, United States of America.
Saida BenomarDepartment of Materials Science & Engineering, Iowa State University, Ames, Iowa, United States of America; Ames Laboratory, US Department of Energy, Iowa State University, Ames, Iowa, United States of America.
Hannah VanEveryDepartment of Materials Science & Engineering, Iowa State University, Ames, Iowa, United States of America; Department of Chemical & Biological Engineering, Iowa State University, Ames, Iowa, United States of America.
Ludovico CademartiriDepartment of Materials Science & Engineering, Iowa State University, Ames, Iowa, United States of America; Ames Laboratory, US Department of Energy, Iowa State University, Ames, Iowa, United States of America; Department of Chemical & Biological Engineering, Iowa State University, Ames, Iowa, United States of America.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We describe a simple, inexpensive, but remarkably versatile and controlled growth environment for the observation of plant germination and seedling root growth on a flat, horizontal surface over periods of weeks. The setup provides to each plant a controlled humidity (between 56% and 91% RH), and contact with both nutrients and atmosphere. The flat and horizontal geometry of the surface supporting the roots eliminates the gravitropic bias on their development and facilitates the imaging of the entire root system. Experiments can be setup under sterile conditions and then transferred to a non-sterile environment. The system can be assembled in 1-2 minutes, costs approximately 8.78$ per plant, is almost entirely reusable (0.43$ per experiment in disposables), and is easily scalable to a variety of plants. We demonstrate the performance of the system by germinating, growing, and imaging Wheat (Triticum aestivum), Corn (Zea mays), and Wisconsin Fast Plants (Brassica rapa). Germination rates were close to those expected for optimal conditions.

Indexed as

Environment, ControlledHumidityBrassica rapaGerminationPlant RootsSeedlingsTriticumZea mays

Identifiers

PMID24806462
PMCPMC4013031

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.