ArticlePloS one2014
Development of an allele-specific PCR assay for simultaneous sero-typing of avian pathogenic Escherichia coli predominant O1, O2, O18 and O78 strains.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 45 citations in OpenAlex.
- Direct interaction between avian pathogenicMicrobiology spectrum · 2026Article
- Bacteriophage cocktail in drinking water suppresses systemic avian pathogenicCurrent research in microbial sciences · 2026Article
- Molecular Characterization and Antibiotic Resistance of Avian PathogenicAnimals : an open access journal from MDPI · 2025Article
- A virulent escherichia coli O121-B2-ST131 strain causes hemorrhagic pneumonia in mink: evidence from pathogenicity and animal challenge experiments.BMC veterinary research · 2025Article
- Molecular Characterization, Antibiotic Resistance, and Biofilm Formation ofMicroorganisms · 2025Article
- Plasma metabolomics of Mycoplasma synoviae infection in SPF White Leghorn hens by liquid chromatography-tandem mass spectrometry.Veterinary research · 2025Article
- A rapid detection of Avian Pathogenic Escherichia coli (APEC) strains based on minimal number of virulence markers identified by whole genome sequencing.BMC microbiology · 2025Article
- Bacteriophages Mediating Effective Elimination of Multidrug-Resistant Avian PathogenicPHAGE (New Rochelle, N.Y.) · 2024Article
- Isolation and identification of specificFrontiers in microbiology · 2024Article
- Co-Occurrence ofAntibiotics (Basel, Switzerland) · 2023Article
- Genetic, Antigenic, and Pathobiological Characterization of H9 and H6 Low Pathogenicity Avian Influenza Viruses Isolated in Vietnam from 2014 to 2018.Microorganisms · 2023Article
- A lytic phage to control multidrug-resistant avian pathogenicFrontiers in cellular and infection microbiology · 2023Article
- Isolation, Molecular Characterization, and Antibiotic Resistance of Avian PathogenicVeterinary sciences · 2022Article
- Investigation of Serotype Prevalence ofVeterinary sciences · 2022Article
- Characteristics, pathogenic mechanism, zoonotic potential, drug resistance, and prevention of avian pathogenicFrontiers in microbiology · 2022Review
- An Integrated Perspective on Virulence-Associated Genes (VAGs), Antimicrobial Resistance (AMR), and Phylogenetic Clusters of Pathogenic and Non-pathogenic AvianFrontiers in veterinary science · 2021Article
- Prevalence and Genetic Relationship of PredominantVeterinary medicine international · 2021Article
- Deletion of theFrontiers in veterinary science · 2021Article
- Exploiting bacterial outer membrane vesicles as a cross-protective vaccine candidate against avian pathogenic Escherichia coli (APEC).Microbial cell factories · 2020Article
- Bacteriophage activity against and characterisation of avian pathogenic Escherichia coli isolated from colibacillosis cases in Uganda.PloS one · 2020Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Systemic infections by avian pathogenic Escherichia coli (APEC) are economically devastating to poultry industries worldwide. E. coli strains belonging to serotypes O1, O2, O18 and O78 are preferentially associated with avian colibacillosis. The rfb gene cluster controlling O antigen synthesis is usually various among different E. coli serotypes. In present study, the rfb gene clusters of E. coli serotypes O1, O2, O18 and O78 were characterized and compared. Based on the serotype-specific genes in rfb gene cluster, an allele-specific polymerase chain reaction (PCR) assay was developed. This PCR assay was highly specific and reliable for sero-typing of APEC O1, O2, O18 and O78 strains. The sensitivity of the assay was determined as 10 pg DNA or 10 colony forming units (CFUs) bacteria for serotypes O2 and O18 strains, and 500 pg DNA or 1,000 CFUs bacteria for serotypes O1 and O78 strains. Using this PCR system, APEC isolates and the infected tissue samples were categorized successfully. Furthermore, it was able to differentiate the serotypes for the samples with multi-agglutination in the traditional serum agglutination assay. Therefore, the allele-specific PCR is more simple, rapid and accurate assay for APEC diagnosis, epidemiologic study and vaccine development.
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