ArticlePloS one2014
Lactobacillus crispatus dominant vaginal microbiome is associated with inhibitory activity of female genital tract secretions against Escherichia coli.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed.
- Soluble Immune Mediators and Vaginal Bacteria Impact Innate Genital Mucosal Antimicrobial Activity in Young Women.American journal of reproductive immunology (New York, N.Y. : 1989) · 2015Trial
- Levels of Genital Tract Defensins and Cytokines Differ between HIV-Uninfected US and African Women.American journal of reproductive immunology (New York, N.Y. : 1989) · 2015Trial
- Review
- Vaginal aging: from concealed symptoms to defined biomarkers.npj aging · 2026Review
- Research Progress on perinatalFrontiers in microbiology · 2026Review
- Divergence in the sow vaginal microbiota is associated with fertility.Reproduction (Cambridge, England) · 2025Article
- The Microbiota-Human Health Axis.Microorganisms · 2025Review
- Modified vaginal lactobacilli expressing fluorescent and luminescent proteins for more effective monitoring of their release from nanofibers, safety and cell adhesion.Microbial cell factories · 2024Article
- The Vaginal Microbiome of Transgender and Gender Nonbinary Individuals.Transgender health · 2024Article
- Vaginal dysbiosis and the potential of vaginal microbiome-directed therapeutics.Frontiers in microbiomes · 2024Review
- Article
- Article
- Comparison of vaginal microbiota between women with inflammatory bowel disease and healthy controls.PloS one · 2023Article
- Human vaginal microbiota colonization is regulated by female sex hormones in a mouse model.Frontiers in cellular and infection microbiology · 2023Article
- Article
- Protective Effect ofPathogens (Basel, Switzerland) · 2022Article
- Longitudinal study of the vaginal microbiome in pregnancies involving preterm labor.Fujita medical journal · 2022Article
- Environmental and Human Microbiome for Health.Life (Basel, Switzerland) · 2022Review
- Engineering of Vaginal Lactobacilli to Express Fluorescent Proteins Enables the Analysis of Their Mixture in Nanofibers.International journal of molecular sciences · 2021Article
- Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors.
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Abstract
objectiveFemale genital tract secretions inhibit E. coli ex vivo and the activity may prevent colonization and provide a biomarker of a healthy microbiome. We hypothesized that high E. coli inhibitory activity would be associated with a Lactobacillus crispatus and/or jensenii dominant microbiome and differ from that of women with low inhibitory activity. STUDY
designVaginal swab cell pellets from 20 samples previously obtained in a cross-sectional study of near-term pregnant and non-pregnant healthy women were selected based on having high (>90% inhibition) or low (<20% inhibition) anti-E. coli activity. The V6 region of the 16S ribosomal RNA gene was amplified and sequenced using the Illumina HiSeq 2000 platform. Filtered culture supernatants from Lactobacillus crispatus, Lactobacillus iners, and Gardnerella vaginalis were also assayed for E. coli inhibitory activity.
resultsSixteen samples (10 with high and 6 with low activity) yielded evaluable microbiome data. There was no difference in the predominant microbiome species in pregnant compared to non-pregnant women (n = 8 each). However, there were significant differences between women with high compared to low E. coli inhibitory activity. High activity was associated with a predominance of L. crispatus (p<0.007) and culture supernatants from L. crispatus exhibited greater E. coli inhibitory activity compared to supernatants obtained from L. iners or G. vaginalis. Notably, the E. coli inhibitory activity varied among different strains of L. crispatus.
conclusionMicrobiome communities with abundant L. crispatus likely contribute to the E. coli inhibitory activity of vaginal secretions and efforts to promote this environment may prevent E. coli colonization and related sequelae including preterm birth.
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