ArticleThe Plant journal : for cell and molecular biology2014
Glycosylinositol phosphorylceramides from Rosa cell cultures are boron-bridged in the plasma membrane and form complexes with rhamnogalacturonan II.
Article in The Plant journal : for cell and molecular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 154 citations in OpenAlex.
- Rhamnogalacturonan-II Dimerisation Reinforces Salt Resistance in Sugar Beet.Plant, cell & environment · 2026Article
- Advanced lipidomic techniques for high-throughput profiling of complex sphingolipids in plant tissues.Journal of plant research · 2026Article
- Effects of exogenous γ-aminobutyric acid concentrations on growth and nutrient uptake in trifoliate orange (Frontiers in plant science · 2026Article
- Complex sphingolipid metabolism impacts cell division and plasmodesmal development in the moss Physcomitrium patens.Plant physiology · 2025Article
- Plasticity of the cytotoxic Nep1-like protein enables promiscuity in binding to its lipid receptor glycosylinositol phosphorylceramides.Science advances · 2025Article
- AAAP gene family evolution and transcriptional regulation in Eucalyptus grandis under nitrogen, phosphate and boron deficiencies.BMC plant biology · 2025Article
- Storming the barricades of rhamnogalacturonan-II synthesis and function.The Plant cell · 2025Review
- Automated mass spectrometry-based profiling of multi-glycosylated glycosyl inositol phospho ceramides (GIPC) reveals specific series GIPC rearrangements during barley grain development and heat stress response.The Plant journal : for cell and molecular biology · 2025Article
- Molecular mechanisms affected by boron deficiency in root and shoot meristems of plants.Journal of experimental botany · 2025Review
- Article
- Transport and regulatory mechanisms of boron in plants.Frontiers in plant science · 2025Review
- Integrative analysis of the transcriptome and proteome reveals the molecular responses of tobacco to boron deficiency.BMC plant biology · 2024Article
- Insights into Plant Sensory Mechanisms under Abiotic Stresses.Plants (Basel, Switzerland) · 2024Review
- Effects of Foliar Boron Application on Physiological and Antioxidants Responses in Highbush Blueberry (Plants (Basel, Switzerland) · 2024Article
- Arabinogalactan-Proteins as Boron-Acting Enzymes, Cross-Linking the Rhamnogalacturonan-II Domains of Pectin.Plants (Basel, Switzerland) · 2023Review
- Leaf Proteomic Analysis in Seedlings of Two Maize Landraces with Different Tolerance to Boron Toxicity.Plants (Basel, Switzerland) · 2023Article
- Chronic Ionizing Radiation of Plants: An Evolutionary Factor from Direct Damage to Non-Target Effects.Plants (Basel, Switzerland) · 2023Review
- Making and breaking of boron bridges in the pectic domain rhamnogalacturonan-II at apoplastic pH in vivo and in vitro.The Plant journal : for cell and molecular biology · 2023Article
- What Can Boron Deficiency Symptoms Tell Us about Its Function and Regulation?Plants (Basel, Switzerland) · 2023Review
- Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Boron (B) is essential for plant cell-wall structure and membrane functions. Compared with its role in cross-linking the pectic domain rhamnogalacturonan II (RG-II), little information is known about the biological role of B in membranes. Here, we investigated the involvement of glycosylinositol phosphorylceramides (GIPCs), major components of lipid rafts, in the membrane requirement for B. Using thin-layer chromatography and mass spectrometry, we first characterized GIPCs from Rosa cell culture. The major GIPC has one hexose residue, one hexuronic acid residue, inositol phosphate, and a ceramide moiety with a C18 trihydroxylated mono-unsaturated long-chain base and a C24 monohydroxylated saturated fatty acid. Disrupting B bridging (by B starvation in vivo or by treatment with cold dilute HCl or with excess borate in vitro) enhanced the GIPCs' extractability. As RG-II is the main B-binding site in plants, we investigated whether it could form a B-centred complex with GIPCs. Using high-voltage paper electrophoresis, we showed that addition of GIPCs decreased the electrophoretic mobility of radiolabelled RG-II, suggesting formation of a GIPC-B-RG-II complex. Last, using polyacrylamide gel electrophoresis, we showed that added GIPCs facilitate RG-II dimerization in vitro. We conclude that B plays a structural role in the plasma membrane. The disruption of membrane components by high borate may account for the phytotoxicity of excess B. Moreover, the in-vitro formation of a GIPC-B-RG-II complex gives the first molecular explanation of the wall-membrane attachment sites observed in vivo. Finally, our results suggest a role for GIPCs in the RG-II dimerization process.
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