ArticlePloS one2014
Manipulation therapy prior to diagnosis induced primary osteosarcoma metastasis--from clinical to basic research.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 25 citations in OpenAlex.
- Harms of massage in persons with cancer or receiving treatment for cancer: A systematic review and meta-analysis.PloS one · 2026Pooled it
- Use of Instrumental Physical Therapies and Manual Therapy in Cancer Patients: A Systematic Review.Cancers · 2026Review
- Lymphatic pumping technique in mice alters blood parameters and metastatic melanoma in an age-dependent manner.Experimental biology and medicine (Maywood, N.J.) · 2026Article
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- Establishing the Philippine Cancer Center National Cancer Research Agenda 2024-2028: Insights and Future Directions.JCO global oncology · 2025Article
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- Importance of Metalloproteinase Enzyme Group in Selected Skeletal System Diseases.International journal of molecular sciences · 2023Review
- Massage manipulation and progression of osteosarcoma, does it really correlate: a combination of prospective and retrospective cohort study.Scientific reports · 2023Observational
- Identification of Lysosome-Associated Protein Transmembrane-4 as a Novel Therapeutic Target for Osteosarcoma Treatment.Orthopaedic surgery · 2020Article
- Onco-Esthetics Dilemma: Is There a Role for Electrocosmetic-Medical Devices?Frontiers in oncology · 2020Review
- Identification and validation of microRNAs and their targets expressed in osteosarcoma.Oncology letters · 2019Article
- Article
- Concurrent Osteosarcoma Theranostic Strategy Using Contrast-Enhanced Ultrasound and Drug-Loaded Bubbles.Pharmaceutics · 2019Article
- Role of estrogen receptors and Src signaling in mechanisms of bone metastasis by estrogen receptor positive breast cancers.Journal of translational medicine · 2017Article
- Outcomes of 50 Patients With Ewing Sarcoma Family of Tumors Treated at a Single Institution in Taiwan.Medicine · 2016Observational
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Osteosarcoma (OS) patients who suffer manipulation therapy (MT) prior to diagnosis resulted in poor prognosis with increasing metastasis or recurrence rate. The aim of the study is to establish an in vivo model to identify the effects of MT on OS. The enrolled 235 OS patients were followed up in this study. In vivo nude mice model with tibia injection of GFP-labeled human OS cells were randomly allocated into MT(+) that with repeated massage on tumor site twice a week and no treatment as MT(-) group. The five-year survival, metastasis and recurrence rates were recorded in clinical subjects. X-ray plainfilm, micro-PET/CT scan, histopathology, serum metalloproteinase 2 (MMP2), metalloproteinase 9 (MMP9) level and human kinase domain insert receptor (KDR) pattern were assayed in mice model. The results showed that patient with MT decreased 5-year survival and higher recurrence or metastasis rate. Compatible with clinical findings, the decreased body weight (30.5 ± 0.65 g) and an increased tumor volume (8.3 ± 1.18 mm3) in MT(+) mice were observed. The increasing signal intensity over lymph node region of hind limb by micro-PET/CT and the tumor cells were detected in lung and bilateral lymph nodes only in MT(+) group. MMP2 (214 ± 9.8 ng/ml) and MMP9 (25.5 ± 1.81 ng/ml) were higher in MT(+) group than in MT(-) group (165 ± 7.8 ng/ml and 16.9 ± 1.40 ng/ml, individually) as well as KDR expression. Taking clinical observations and in vivo evidence together, MT treatment leads to poor prognosis of primary osteosarcoma; physicians should pay more attention on patients who seek MT before diagnosis.
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